Linked Life Data

17959646

Source:http://linkedlifedata.com/resource/pubmed/id/17959646

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pubmed-article:17959646pubmed:abstractTextCaulobacter crescentus is widely used as a powerful model system for the study of prokaryotic cell biology and development. Analysis of this organism is complicated by a limited selection of tools for genetic manipulation and inducible gene expression. This study reports the identification and functional characterization of a vanillate-regulated promoter (P(van)) which meets all requirements for application as a multi-purpose expression system in Caulobacter, thus complementing the established xylose-inducible system (P(xyl)). Furthermore, we introduce a newly constructed set of integrating and replicating shuttle vectors that considerably facilitate cell biological and physiological studies in Caulobacter. Based on different narrow and broad-host range replicons, they offer a wide choice of promoters, resistance genes, and fusion partners for the construction of fluorescently or affinity-tagged proteins. Since many of these constructs are also suitable for use in other bacteria, this work provides a comprehensive collection of tools that will enrich many areas of microbiological research.lld:pubmed
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pubmed-article:17959646pubmed:issn1362-4962lld:pubmed
pubmed-article:17959646pubmed:authorpubmed-author:ThanbichlerMa...lld:pubmed
pubmed-article:17959646pubmed:authorpubmed-author:ShapiroLucyLlld:pubmed
pubmed-article:17959646pubmed:authorpubmed-author:IniestaAntoni...lld:pubmed
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pubmed-article:17959646pubmed:volume35lld:pubmed
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pubmed-article:17959646pubmed:dateRevised2010-9-16lld:pubmed
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pubmed-article:17959646pubmed:year2007lld:pubmed
pubmed-article:17959646pubmed:articleTitleA comprehensive set of plasmids for vanillate- and xylose-inducible gene expression in Caulobacter crescentus.lld:pubmed
pubmed-article:17959646pubmed:affiliationMax Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Strasse, 35043 Marburg, Germany. thanbichler@mpi-marburg.mpg.delld:pubmed
pubmed-article:17959646pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:17959646pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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