| pubmed-article:1793293 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1793293 | lifeskim:mentions | umls-concept:C0003232 | lld:lifeskim |
| pubmed-article:1793293 | lifeskim:mentions | umls-concept:C1527148 | lld:lifeskim |
| pubmed-article:1793293 | lifeskim:mentions | umls-concept:C0442335 | lld:lifeskim |
| pubmed-article:1793293 | pubmed:issue | 11 | lld:pubmed |
| pubmed-article:1793293 | pubmed:dateCreated | 1992-4-2 | lld:pubmed |
| pubmed-article:1793293 | pubmed:abstractText | The integrative vectors pSU 475 and pSU 476 with variable numbers of copies per genome were developed for antibiotic producing actinomycetes. For this, the amplifying sequence AUD-Sr 1 of Streptomyces rimosus and the BamHIB fragment of the eSA 1 genetic element from Streptomyces antibioticus were used. The eSA 1 fragment was an element required for integration of a vector to the actinomycete chromosomes since it was homologous with the chromosomal DNAs of S. lividans, S. erythraeus and S. antibioticus. At the first stage the AUD-Sr 1 sequence within the actinomycete plastid pSU 23 was cloned by the vector pUC 19 to E coli. In that experiment the 12.4-kb plasmid pSU 449 was isolated. At the second stage the BamHIB-fragment of the eSA 1 element was incorporated into the resultant hybrid plasmid pSU 449. The 16.5-kb hybrid plasmids pSU 475 and pSU 476 were isolated. In these plasmids the BamHIB fragment of eSA 1 was present in two orientations. The developed vectors were useful in cloning DNA to S. lividans and S. erythraeus. | lld:pubmed |
| pubmed-article:1793293 | pubmed:language | rus | lld:pubmed |
| pubmed-article:1793293 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1793293 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1793293 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1793293 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1793293 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:1793293 | pubmed:issn | 0235-2990 | lld:pubmed |
| pubmed-article:1793293 | pubmed:author | pubmed-author:DanilenkoV... | lld:pubmed |
| pubmed-article:1793293 | pubmed:author | pubmed-author:Grigor'evA... | lld:pubmed |
| pubmed-article:1793293 | pubmed:author | pubmed-author:ZhukovD BDB | lld:pubmed |
| pubmed-article:1793293 | pubmed:author | pubmed-author:OrlovaV AVA | lld:pubmed |
| pubmed-article:1793293 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1793293 | pubmed:volume | 36 | lld:pubmed |
| pubmed-article:1793293 | pubmed:geneSymbol | AUD-Sr1 | lld:pubmed |
| pubmed-article:1793293 | pubmed:geneSymbol | eSA1 | lld:pubmed |
| pubmed-article:1793293 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1793293 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1793293 | pubmed:pagination | 3-5 | lld:pubmed |
| pubmed-article:1793293 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:1793293 | pubmed:meshHeading | pubmed-meshheading:1793293-... | lld:pubmed |
| pubmed-article:1793293 | pubmed:meshHeading | pubmed-meshheading:1793293-... | lld:pubmed |
| pubmed-article:1793293 | pubmed:meshHeading | pubmed-meshheading:1793293-... | lld:pubmed |
| pubmed-article:1793293 | pubmed:meshHeading | pubmed-meshheading:1793293-... | lld:pubmed |
| pubmed-article:1793293 | pubmed:meshHeading | pubmed-meshheading:1793293-... | lld:pubmed |
| pubmed-article:1793293 | pubmed:meshHeading | pubmed-meshheading:1793293-... | lld:pubmed |
| pubmed-article:1793293 | pubmed:meshHeading | pubmed-meshheading:1793293-... | lld:pubmed |
| pubmed-article:1793293 | pubmed:meshHeading | pubmed-meshheading:1793293-... | lld:pubmed |
| pubmed-article:1793293 | pubmed:year | 1991 | lld:pubmed |
| pubmed-article:1793293 | pubmed:articleTitle | [Development of integrative vectors for Actinomycetes--producers of antibiotics]. | lld:pubmed |
| pubmed-article:1793293 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1793293 | pubmed:publicationType | English Abstract | lld:pubmed |
