| pubmed-article:17929575 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:17929575 | lifeskim:mentions | umls-concept:C0004611 | lld:lifeskim |
| pubmed-article:17929575 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:17929575 | lifeskim:mentions | umls-concept:C0014834 | lld:lifeskim |
| pubmed-article:17929575 | lifeskim:mentions | umls-concept:C0317981 | lld:lifeskim |
| pubmed-article:17929575 | lifeskim:mentions | umls-concept:C0009015 | lld:lifeskim |
| pubmed-article:17929575 | lifeskim:mentions | umls-concept:C0001128 | lld:lifeskim |
| pubmed-article:17929575 | lifeskim:mentions | umls-concept:C0205419 | lld:lifeskim |
| pubmed-article:17929575 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:17929575 | lifeskim:mentions | umls-concept:C0051581 | lld:lifeskim |
| pubmed-article:17929575 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:17929575 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:17929575 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:17929575 | pubmed:dateCreated | 2007-10-12 | lld:pubmed |
| pubmed-article:17929575 | pubmed:abstractText | The gene coding for glutaryl-7-aminocephalosporic acid acylase (Gl7ACA acylase) of the bacterium Brevundimonas diminuta (BrdGl7ACA), a commercial enzyme widely used in modem biocatalytic technologies for manufacture of b-lactam antibiotics, was cloned. Efficient expression systems for producing a "native" recombinant BrdGl7ACA and its analogs modified by attaching affinity groups--the chitin-binding domain of chitinases A1 and hexahistidine sequence--were designed. It was demonstrated that both the recombinant hybrid proteins and the native Gl7ACA acylase produced in E. coli cells underwent a correct autoproteolytic processing with generation of functionally active enzymes and could be isolated with a high yield using one-step affinity chromatography. | lld:pubmed |
| pubmed-article:17929575 | pubmed:language | rus | lld:pubmed |
| pubmed-article:17929575 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17929575 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:17929575 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17929575 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17929575 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17929575 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17929575 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17929575 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17929575 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:17929575 | pubmed:issn | 0555-1099 | lld:pubmed |
| pubmed-article:17929575 | pubmed:author | pubmed-author:SkriabinK GKG | lld:pubmed |
| pubmed-article:17929575 | pubmed:author | pubmed-author:El'darovM AMA | lld:pubmed |
| pubmed-article:17929575 | pubmed:author | pubmed-author:LopatinS ASA | lld:pubmed |
| pubmed-article:17929575 | pubmed:author | pubmed-author:KhatuntsevaS... | lld:pubmed |
| pubmed-article:17929575 | pubmed:author | pubmed-author:Ze?nalovO AOA | lld:pubmed |
| pubmed-article:17929575 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:17929575 | pubmed:volume | 43 | lld:pubmed |
| pubmed-article:17929575 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:17929575 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:17929575 | pubmed:pagination | 462-70 | lld:pubmed |
| pubmed-article:17929575 | pubmed:meshHeading | pubmed-meshheading:17929575... | lld:pubmed |
| pubmed-article:17929575 | pubmed:meshHeading | pubmed-meshheading:17929575... | lld:pubmed |
| pubmed-article:17929575 | pubmed:meshHeading | pubmed-meshheading:17929575... | lld:pubmed |
| pubmed-article:17929575 | pubmed:meshHeading | pubmed-meshheading:17929575... | lld:pubmed |
| pubmed-article:17929575 | pubmed:meshHeading | pubmed-meshheading:17929575... | lld:pubmed |
| pubmed-article:17929575 | pubmed:meshHeading | pubmed-meshheading:17929575... | lld:pubmed |
| pubmed-article:17929575 | pubmed:meshHeading | pubmed-meshheading:17929575... | lld:pubmed |
| pubmed-article:17929575 | pubmed:meshHeading | pubmed-meshheading:17929575... | lld:pubmed |
| pubmed-article:17929575 | pubmed:articleTitle | [Cloning and expression of variants of the glutaryl-7-aminocephalosporic acid acylase of the bacterium Brevundimonas diminuta in Escherichia coli cells]. | lld:pubmed |
| pubmed-article:17929575 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:17929575 | pubmed:publicationType | English Abstract | lld:pubmed |
