| pubmed-article:17922505 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:17922505 | lifeskim:mentions | umls-concept:C0041942 | lld:lifeskim |
| pubmed-article:17922505 | lifeskim:mentions | umls-concept:C0169964 | lld:lifeskim |
| pubmed-article:17922505 | lifeskim:mentions | umls-concept:C1979842 | lld:lifeskim |
| pubmed-article:17922505 | lifeskim:mentions | umls-concept:C0812409 | lld:lifeskim |
| pubmed-article:17922505 | lifeskim:mentions | umls-concept:C0013862 | lld:lifeskim |
| pubmed-article:17922505 | lifeskim:mentions | umls-concept:C0185125 | lld:lifeskim |
| pubmed-article:17922505 | lifeskim:mentions | umls-concept:C1704732 | lld:lifeskim |
| pubmed-article:17922505 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
| pubmed-article:17922505 | pubmed:issue | 21 | lld:pubmed |
| pubmed-article:17922505 | pubmed:dateCreated | 2007-10-31 | lld:pubmed |
| pubmed-article:17922505 | pubmed:abstractText | The 40-60 pituitary human growth hormone (hGH) isoforms are so similar in their physico-chemical properties (charge, size, hydrophobicity) that the limited resolutions of chromatographic separation methodologies have not permitted most of them to be isolated. However, application of high-resolution preparative alkaline urea gradient PAGE has facilitated isolation of a disulfide-linked mercaptoethanol-resistant (MER) 45 kDa hGH dimer. Human pituitary extracts were separated by Sephadex G-100 chromatography under alkaline conditions. Pooled fractions containing MER-45 kDa hGH, as determined by SDS-PAGE, were then separated by Sephadex G-100 chromatography under acidic conditions followed by diethylaminoethyl (DEAE) anion-exchange chromatography. Pooled DEAE fractions containing MER-45 kDa hGH and other hGH isoforms were then separated by preparative electrophoresis in an alkaline polyacrylamide gradient (5-20%) slab gel containing 8 M urea into five distinct protein zones. One electroeluted zone contained pure MER-45 kDa hGH. The dimeric hGH isoform was immunoreactive at low concentrations (effective dose to produce 50% response (ED(50)) +/- S.E. = 58 +/- 5.00 pM) in a hGH radioimmunoassay, similar to that of standard monomeric hGH (ED(50) +/- S.E. = 22.93 +/- 3.90 pM), indicating that is was conformationally intact. Alkaline urea gradient PAGE is a valuable tool for preparative separation of structurally similar proteins such as isoforms of the hGH family. | lld:pubmed |
| pubmed-article:17922505 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17922505 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17922505 | pubmed:language | eng | lld:pubmed |
| pubmed-article:17922505 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17922505 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:17922505 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17922505 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17922505 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17922505 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17922505 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:17922505 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:17922505 | pubmed:issn | 0173-0835 | lld:pubmed |
| pubmed-article:17922505 | pubmed:author | pubmed-author:HaroLuis SLS | lld:pubmed |
| pubmed-article:17922505 | pubmed:author | pubmed-author:GrigorianAlex... | lld:pubmed |
| pubmed-article:17922505 | pubmed:author | pubmed-author:BustamanteJua... | lld:pubmed |
| pubmed-article:17922505 | pubmed:author | pubmed-author:MartinezAndre... | lld:pubmed |
| pubmed-article:17922505 | pubmed:author | pubmed-author:AguilarRobert... | lld:pubmed |
| pubmed-article:17922505 | pubmed:author | pubmed-author:MuñozJesusJ | lld:pubmed |
| pubmed-article:17922505 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:17922505 | pubmed:volume | 28 | lld:pubmed |
| pubmed-article:17922505 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:17922505 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:17922505 | pubmed:pagination | 3829-36 | lld:pubmed |
| pubmed-article:17922505 | pubmed:meshHeading | pubmed-meshheading:17922505... | lld:pubmed |
| pubmed-article:17922505 | pubmed:meshHeading | pubmed-meshheading:17922505... | lld:pubmed |
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| pubmed-article:17922505 | pubmed:meshHeading | pubmed-meshheading:17922505... | lld:pubmed |
| pubmed-article:17922505 | pubmed:meshHeading | pubmed-meshheading:17922505... | lld:pubmed |
| pubmed-article:17922505 | pubmed:meshHeading | pubmed-meshheading:17922505... | lld:pubmed |
| pubmed-article:17922505 | pubmed:meshHeading | pubmed-meshheading:17922505... | lld:pubmed |
| pubmed-article:17922505 | pubmed:meshHeading | pubmed-meshheading:17922505... | lld:pubmed |
| pubmed-article:17922505 | pubmed:meshHeading | pubmed-meshheading:17922505... | lld:pubmed |
| pubmed-article:17922505 | pubmed:meshHeading | pubmed-meshheading:17922505... | lld:pubmed |
| pubmed-article:17922505 | pubmed:meshHeading | pubmed-meshheading:17922505... | lld:pubmed |
| pubmed-article:17922505 | pubmed:year | 2007 | lld:pubmed |
| pubmed-article:17922505 | pubmed:articleTitle | Preparative alkaline urea gradient PAGE: application to purification of extraordinarily-stable disulfide-linked homodimer of human growth hormone. | lld:pubmed |
| pubmed-article:17922505 | pubmed:affiliation | Department of Biochemistry and Molecular Genetics, University of Alabama School of Medicine, Birmingham, AL, USA. | lld:pubmed |
| pubmed-article:17922505 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:17922505 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
| entrez-gene:2688 | entrezgene:pubmed | pubmed-article:17922505 | lld:entrezgene |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:17922505 | lld:entrezgene |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17922505 | lld:pubmed |
