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pubmed-article:17914459pubmed:abstractTextTranscription by RNA polymerase II is accompanied by dynamic changes in chromatin, including the eviction/deposition of nucleosomes or the covalent modification of histone subunits. This study examined the role of the histone H3/H4 chaperones, Asf1 and HIR, in histone mobility during transcription, with particular focus on the histone exchange pathway, using a dual histone expression system. The results showed that the exchange of H3/H4 normally occurs during transcription by the histone chaperones. Both Asf1 and HIR are important for histone deposition but have a different effect on histone exchange. While Asf1 mediated incorporation of external H3/H4 and renewal of pre-existing histones, HIR opposed it. The balance of two opposing activities might be an important mechanism for determining current chromatin states.lld:pubmed
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pubmed-article:17914459pubmed:authorpubmed-author:HanJeung-Whan...lld:pubmed
pubmed-article:17914459pubmed:authorpubmed-author:ChoEun-JungEJlld:pubmed
pubmed-article:17914459pubmed:authorpubmed-author:KimHye-JinHJlld:pubmed
pubmed-article:17914459pubmed:authorpubmed-author:YounHong-DukH...lld:pubmed
pubmed-article:17914459pubmed:authorpubmed-author:SeolJa-HwanJHlld:pubmed
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pubmed-article:17914459pubmed:articleTitleHistone chaperones regulate histone exchange during transcription.lld:pubmed
pubmed-article:17914459pubmed:affiliationCollege of Pharmacy, Sungkyunkwan University, Suwon, Gyeonggi-do, Republic of Korea.lld:pubmed
pubmed-article:17914459pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:17914459pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed