| pubmed-article:17914059 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:17914059 | lifeskim:mentions | umls-concept:C0015283 | lld:lifeskim |
| pubmed-article:17914059 | lifeskim:mentions | umls-concept:C0084697 | lld:lifeskim |
| pubmed-article:17914059 | lifeskim:mentions | umls-concept:C0300824 | lld:lifeskim |
| pubmed-article:17914059 | lifeskim:mentions | umls-concept:C1704675 | lld:lifeskim |
| pubmed-article:17914059 | lifeskim:mentions | umls-concept:C1444748 | lld:lifeskim |
| pubmed-article:17914059 | lifeskim:mentions | umls-concept:C0596235 | lld:lifeskim |
| pubmed-article:17914059 | lifeskim:mentions | umls-concept:C0086597 | lld:lifeskim |
| pubmed-article:17914059 | lifeskim:mentions | umls-concept:C0183362 | lld:lifeskim |
| pubmed-article:17914059 | lifeskim:mentions | umls-concept:C0851827 | lld:lifeskim |
| pubmed-article:17914059 | lifeskim:mentions | umls-concept:C1701901 | lld:lifeskim |
| pubmed-article:17914059 | lifeskim:mentions | umls-concept:C0205224 | lld:lifeskim |
| pubmed-article:17914059 | pubmed:issue | 12 | lld:pubmed |
| pubmed-article:17914059 | pubmed:dateCreated | 2007-11-28 | lld:pubmed |
| pubmed-article:17914059 | pubmed:abstractText | Synaptotagmins contain tandem C2 domains and function as Ca(2+) sensors for vesicle exocytosis but the mechanism for coupling Ca(2+) rises to membrane fusion remains undefined. Synaptotagmins bind SNAREs, essential components of the membrane fusion machinery, but the role of these interactions in Ca(2+)-triggered vesicle exocytosis has not been directly assessed. We identified sites on synaptotagmin-1 that mediate Ca(2+)-dependent SNAP25 binding by zero-length cross-linking. Mutation of these sites in C2A and C2B eliminated Ca(2+)-dependent synaptotagmin-1 binding to SNAREs without affecting Ca(2+)-dependent membrane binding. The mutants failed to confer Ca(2+) regulation on SNARE-dependent liposome fusion and failed to restore Ca(2+)-triggered vesicle exocytosis in synaptotagmin-deficient PC12 cells. The results provide direct evidence that Ca(2+)-dependent SNARE binding by synaptotagmin is essential for Ca(2+)-triggered vesicle exocytosis and that Ca(2+)-dependent membrane binding by itself is insufficient to trigger fusion. A structure-based model of the SNARE-binding surface of C2A provided a new view of how Ca(2+)-dependent SNARE and membrane binding occur simultaneously. | lld:pubmed |
| pubmed-article:17914059 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:language | eng | lld:pubmed |
| pubmed-article:17914059 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:17914059 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17914059 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:17914059 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:17914059 | pubmed:issn | 1059-1524 | lld:pubmed |
| pubmed-article:17914059 | pubmed:author | pubmed-author:MitchellJ CJC | lld:pubmed |
| pubmed-article:17914059 | pubmed:author | pubmed-author:LynchK LKL | lld:pubmed |
| pubmed-article:17914059 | pubmed:author | pubmed-author:LarsenE CEC | lld:pubmed |
| pubmed-article:17914059 | pubmed:author | pubmed-author:MartinT F JTF | lld:pubmed |
