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pubmed-article:17905859pubmed:abstractTextTo isolate novel auxin-responsive mutants in Arabidopsis (Arabidopsis thaliana), we screened mutants for root growth resistance to a putative antiauxin, p-chlorophenoxyisobutyric acid (PCIB), which inhibits auxin action by interfering the upstream auxin-signaling events. Eleven PCIB-resistant mutants were obtained. Genetic mapping indicates that the mutations are located in at least five independent loci, including two known auxin-related loci, TRANSPORT INHIBITOR RESPONSE1 and Arabidopsis CULLIN1. antiauxin-resistant mutants (aars) aar3-1, aar4, and aar5 were also resistant to 2,4-dichlorophenoxyacetic acid as shown by a root growth assay. Positional cloning of aar3-1 revealed that the AAR3 gene encodes a protein with a domain of unknown function (DUF298), which has not previously been implicated in auxin signaling. The protein has a putative nuclear localization signal and shares homology with the DEFECTIVE IN CULLIN NEDDYLATION-1 protein through the DUF298 domain. The results also indicate that PCIB can facilitate the identification of factors involved in auxin or auxin-related signaling.lld:pubmed
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pubmed-article:17905859pubmed:authorpubmed-author:TanakaAtsushi...lld:pubmed
pubmed-article:17905859pubmed:authorpubmed-author:MiyazakiYujiYlld:pubmed
pubmed-article:17905859pubmed:authorpubmed-author:NarumiIssayIlld:pubmed
pubmed-article:17905859pubmed:authorpubmed-author:KoshibaTomoka...lld:pubmed
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pubmed-article:17905859pubmed:authorpubmed-author:RahmanAbidurAlld:pubmed
pubmed-article:17905859pubmed:authorpubmed-author:HiguchiKanako...lld:pubmed
pubmed-article:17905859pubmed:authorpubmed-author:KiyosueTomohi...lld:pubmed
pubmed-article:17905859pubmed:authorpubmed-author:BiswasKamal...lld:pubmed
pubmed-article:17905859pubmed:authorpubmed-author:Van...lld:pubmed
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pubmed-article:17905859pubmed:pagination773-85lld:pubmed
pubmed-article:17905859pubmed:dateRevised2009-11-18lld:pubmed
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