Linked Life Data

17894875

Source:http://linkedlifedata.com/resource/pubmed/id/17894875

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pubmed-article:17894875pubmed:abstractTextIterative DNA "resequencing" on oligonucleotide microarrays offers a high-throughput method to measure intraspecific iodiversity, one that is especially suited to SNP-dense gene regions such as vertebrate mitochondrial (mtDNA) genomes. However, costs of single-species design and microarray fabrication are prohibitive. A cost-effective, multi-species strategy is to hybridize experimental DNAs from diverse species to a common microarray that is tiled with oligonucleotide sets from multiple, homologous reference genomes. Such a strategy requires that cross-hybridization between the experimental DNAs and reference oligos from the different species not interfere with the accurate recovery of species-specific data. To determine the pattern and limits of such interspecific hybridization, we compared the efficiency of sequence recovery and accuracy of SNP identification by a 15,452-base human-specific microarray challenged with human, chimpanzee, gorilla, and codfish mtDNA genomes.lld:pubmed
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pubmed-article:17894875pubmed:articleTitleInterspecies hybridization on DNA resequencing microarrays: efficiency of sequence recovery and accuracy of SNP detection in human, ape, and codfish mitochondrial DNA genomes sequenced on a human-specific MitoChip.lld:pubmed
pubmed-article:17894875pubmed:affiliationGenetics, Evolution, & Molecular Systematics Laboratory, Department of Biology, Memorial University of Newfoundland, St, John's, NL A1B3X9, Canada. sarahf@mun.calld:pubmed
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