| pubmed-article:17786543 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:17786543 | lifeskim:mentions | umls-concept:C0205474 | lld:lifeskim |
| pubmed-article:17786543 | lifeskim:mentions | umls-concept:C0225897 | lld:lifeskim |
| pubmed-article:17786543 | lifeskim:mentions | umls-concept:C1882687 | lld:lifeskim |
| pubmed-article:17786543 | lifeskim:mentions | umls-concept:C0031640 | lld:lifeskim |
| pubmed-article:17786543 | lifeskim:mentions | umls-concept:C0034318 | lld:lifeskim |
| pubmed-article:17786543 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:17786543 | pubmed:issue | 1-2 | lld:pubmed |
| pubmed-article:17786543 | pubmed:dateCreated | 2007-10-22 | lld:pubmed |
| pubmed-article:17786543 | pubmed:abstractText | In the present study we investigate the biochemical properties of the members of NPP family in synaptosomes prepared from rat heart left ventricles. Using p-nitrophenyl-5'-thymidine monophosphate (p-Nph-5'-TMP) as substrate for E-NPPs in rat cardiac synaptosomes, we observed an alkaline pH dependence, divalent cation dependence and the K ( M ) value corresponded to 91.42 +/- 13.97 microM and the maximal velocity (V ( max )) value calculated was 63.79 +/- 3.59 nmol p-nitrophenol released/min/mg of protein (mean +/- SD, n = 4). Levamisole (1 mM), was ineffective as inhibitor of p-Nph-5'-TMP hydrolysis in pH 8.9 (optimum pH for the enzyme characterized). Suramin (0.25 mM) strongly reduced the hydrolysis of p-Nph-5'-TMP by about 46%. Sodium azide (10 and 20 mM) and gadolinium chloride (0.3 and 0.5 mM), E-NTPases inhibitors, had no effects on p-Nph-5'-TMP hydrolysis. RT-PCR analysis of left ventricle demonstrated the expression of NPP2 and NPP3 enzymes, but excluded the presence of NPP1 member. By quantitative real-time PCR we identified the NPP3 as the enzyme with the highest expression in rat left ventricle. The demonstration of the presence of the E-NPP family in cardiac system, suggest that these enzymes could contribute with the fine-tuning control of the nucleotide levels at the nerve terminal endings of left ventricles that are involved in several cardiac pathologies. | lld:pubmed |
| pubmed-article:17786543 | pubmed:language | eng | lld:pubmed |
| pubmed-article:17786543 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17786543 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:17786543 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17786543 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17786543 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17786543 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17786543 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17786543 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17786543 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17786543 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17786543 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17786543 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17786543 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17786543 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17786543 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:17786543 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:17786543 | pubmed:issn | 0300-8177 | lld:pubmed |
| pubmed-article:17786543 | pubmed:author | pubmed-author:SarkisJoão... | lld:pubmed |
| pubmed-article:17786543 | pubmed:author | pubmed-author:LibermannTowi... | lld:pubmed |
| pubmed-article:17786543 | pubmed:author | pubmed-author:ZerbiniLuiz... | lld:pubmed |
| pubmed-article:17786543 | pubmed:author | pubmed-author:WinkMárcia... | lld:pubmed |
| pubmed-article:17786543 | pubmed:author | pubmed-author:RückerBárbara... | lld:pubmed |
| pubmed-article:17786543 | pubmed:author | pubmed-author:AlmeidaManoel... | lld:pubmed |
| pubmed-article:17786543 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:17786543 | pubmed:volume | 306 | lld:pubmed |
| pubmed-article:17786543 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:17786543 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:17786543 | pubmed:pagination | 247-54 | lld:pubmed |
| pubmed-article:17786543 | pubmed:dateRevised | 2011-7-15 | lld:pubmed |
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| pubmed-article:17786543 | pubmed:meshHeading | pubmed-meshheading:17786543... | lld:pubmed |
| pubmed-article:17786543 | pubmed:year | 2007 | lld:pubmed |
| pubmed-article:17786543 | pubmed:articleTitle | Biochemical characterization of ecto-nucleotide pyrophosphatase/phosphodiesterase (E-NPP, E.C. 3.1.4.1) from rat heart left ventricle. | lld:pubmed |
| pubmed-article:17786543 | pubmed:affiliation | Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul - UFRGS, Rua Ramiro Barcelos, 2600 - Prédio ANEXO, Porto Alegre, RS CEP 90035-003, Brazil. | lld:pubmed |
| pubmed-article:17786543 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:17786543 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17786543 | lld:pubmed |
