| pubmed-article:17722881 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:17722881 | lifeskim:mentions | umls-concept:C1551714 | lld:lifeskim |
| pubmed-article:17722881 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:17722881 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:17722881 | lifeskim:mentions | umls-concept:C1521871 | lld:lifeskim |
| pubmed-article:17722881 | lifeskim:mentions | umls-concept:C1710360 | lld:lifeskim |
| pubmed-article:17722881 | pubmed:issue | 19 | lld:pubmed |
| pubmed-article:17722881 | pubmed:dateCreated | 2007-9-28 | lld:pubmed |
| pubmed-article:17722881 | pubmed:abstractText | Aptamer-based rolling circle amplification (aptamer-RCA) was developed as a novel versatile electrochemical platform for ultrasensitive detection of protein. This method utilized antibodies immobilized on the electrode surface to capture the protein target, and the surface-captured protein was then sandwiched by an aptamer-primer complex. The aptamer-primer sequence mediated an in situ RCA reaction that generated hundreds of copies of a circular DNA template. Detection of the amplified copies via enzymatic silver deposition then allowed enormous sensitivity enhancement in the assay of target protein. This novel aptamer-primer design circumvented time-consuming preparation of the antibody-DNA conjugate for the common immuno-RCA assay. Moreover, the detection strategy based on enzymatic silver deposition enabled a highly efficient readout of the RCA product as compared to a redox-labeled probe based procedure that might exhibit low detection efficiency due to RCA product distance from the electrode. With the platelet-derived growth factor B-chain (PDGF-BB) as a model target, it was demonstrated that the presented method was highly sensitive and specific with a wide detection range of 4 orders of magnitude and a detection limit as low as 10 fM. Because of the wide availability of aptamers for numerous proteins, this platform holds great promise in ultrasensitive immunoassay. | lld:pubmed |
| pubmed-article:17722881 | pubmed:language | eng | lld:pubmed |
| pubmed-article:17722881 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17722881 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:17722881 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17722881 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17722881 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17722881 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17722881 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17722881 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17722881 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:17722881 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:17722881 | pubmed:issn | 0003-2700 | lld:pubmed |
| pubmed-article:17722881 | pubmed:author | pubmed-author:Guo-LiShenS | lld:pubmed |
| pubmed-article:17722881 | pubmed:author | pubmed-author:Ru-QinYuY | lld:pubmed |
| pubmed-article:17722881 | pubmed:author | pubmed-author:ChuXiaX | lld:pubmed |
| pubmed-article:17722881 | pubmed:author | pubmed-author:LongZhouZ | lld:pubmed |
| pubmed-article:17722881 | pubmed:author | pubmed-author:OuLi-JuanLJ | lld:pubmed |
| pubmed-article:17722881 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:17722881 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:17722881 | pubmed:volume | 79 | lld:pubmed |
| pubmed-article:17722881 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:17722881 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:17722881 | pubmed:pagination | 7492-500 | lld:pubmed |
| pubmed-article:17722881 | pubmed:meshHeading | pubmed-meshheading:17722881... | lld:pubmed |
| pubmed-article:17722881 | pubmed:meshHeading | pubmed-meshheading:17722881... | lld:pubmed |
| pubmed-article:17722881 | pubmed:meshHeading | pubmed-meshheading:17722881... | lld:pubmed |
| pubmed-article:17722881 | pubmed:meshHeading | pubmed-meshheading:17722881... | lld:pubmed |
| pubmed-article:17722881 | pubmed:meshHeading | pubmed-meshheading:17722881... | lld:pubmed |
| pubmed-article:17722881 | pubmed:meshHeading | pubmed-meshheading:17722881... | lld:pubmed |
| pubmed-article:17722881 | pubmed:meshHeading | pubmed-meshheading:17722881... | lld:pubmed |
| pubmed-article:17722881 | pubmed:meshHeading | pubmed-meshheading:17722881... | lld:pubmed |
| pubmed-article:17722881 | pubmed:meshHeading | pubmed-meshheading:17722881... | lld:pubmed |
| pubmed-article:17722881 | pubmed:year | 2007 | lld:pubmed |
| pubmed-article:17722881 | pubmed:articleTitle | Aptamer-based rolling circle amplification: a platform for electrochemical detection of protein. | lld:pubmed |
| pubmed-article:17722881 | pubmed:affiliation | State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, P. R. China. | lld:pubmed |
| pubmed-article:17722881 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:17722881 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17722881 | lld:pubmed |
