Biocatalytic microcontact printing.

Source:http://linkedlifedata.com/resource/pubmed/id/17705546

J. Org. Chem. 2007 Sep 14 72 19 7459-61

Download in:

View as

General Info

Authors

Toone EJ, Clark RL, Snyder PW, Johannes MS, Vogen BN

Affiliation

Department of Chemistry, Duke University, Durham, NC 27708-0346, USA.

Abstract

Immobilized biocatalytic lithography is presented as an application of soft lithography. In traditional microcontact printing, diffusion limits resolution of pattern transfer. By using an immobilized catalyst, the lateral resolution of microcontact printing would depend only on the length and flexibility of the tether (<2 nm) as opposed to diffusion (>100 nm). In the work, exonuclease reversibly immobilized on a relief-patterned stamp is used to ablate ssDNA monolayers Percent of ablation was determined via confocal fluorescence microscopy to be approximately 70%.

PMID
17705546

Publication types

Research Support, U.S. Gov't, Non-P.H.S.; Research Support, N.I.H., Extramural