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pubmed-article:17687552pubmed:dateCreated2007-10-24lld:pubmed
pubmed-article:17687552pubmed:abstractTextA novel dichloromethane (DCM)-degrading bacterial strain named WZ-12 (GenBank accession no. EF100968) was isolated and identified as Bacillus circulans based on standard morphological and physiological properties and nucleotide sequence analysis of enzymatically amplified 16S ribosomal deoxyribonucleic acid. DCM dehalogenase from B. circulans WZ-12 was purified to 8.27-fold with a yield of 34.83%. The electrophoretically homogeneous-purified enzyme exhibited a specific activity of 118.82 U/mg. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of purified DCM dehalogenase gave a distinct band with an estimated molecular mass of 20,000 +/- 1,000.lld:pubmed
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pubmed-article:17687552pubmed:authorpubmed-author:ZhangL LLLlld:pubmed
pubmed-article:17687552pubmed:authorpubmed-author:WangJ DJDlld:pubmed
pubmed-article:17687552pubmed:authorpubmed-author:ChenJ MJMlld:pubmed
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pubmed-article:17687552pubmed:volume76lld:pubmed
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pubmed-article:17687552pubmed:pagination1289-96lld:pubmed
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pubmed-article:17687552pubmed:year2007lld:pubmed
pubmed-article:17687552pubmed:articleTitleBacillus circulans WZ-12 - a newly discovered aerobic dichloromethane-degrading methylotrophic bacterium.lld:pubmed
pubmed-article:17687552pubmed:affiliationCollege of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, People's Republic of China.lld:pubmed
pubmed-article:17687552pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:17687552pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed