| pubmed-article:17685239 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:17685239 | lifeskim:mentions | umls-concept:C0006901 | lld:lifeskim |
| pubmed-article:17685239 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:17685239 | lifeskim:mentions | umls-concept:C0016315 | lld:lifeskim |
| pubmed-article:17685239 | lifeskim:mentions | umls-concept:C1948027 | lld:lifeskim |
| pubmed-article:17685239 | lifeskim:mentions | umls-concept:C0201699 | lld:lifeskim |
| pubmed-article:17685239 | lifeskim:mentions | umls-concept:C0936012 | lld:lifeskim |
| pubmed-article:17685239 | lifeskim:mentions | umls-concept:C1524063 | lld:lifeskim |
| pubmed-article:17685239 | lifeskim:mentions | umls-concept:C0074992 | lld:lifeskim |
| pubmed-article:17685239 | lifeskim:mentions | umls-concept:C0522503 | lld:lifeskim |
| pubmed-article:17685239 | pubmed:issue | 1-2 | lld:pubmed |
| pubmed-article:17685239 | pubmed:dateCreated | 2007-8-9 | lld:pubmed |
| pubmed-article:17685239 | pubmed:abstractText | C18-sphingosine 1-phosphate (C18-SPP) is a sphingolipid with important functional and structural roles in cells. In this paper we report a new capillary electrophoresis technique that is coupled to a laser-induced fluorescence detection (CE-LIF) method to quantify the level of C18-SPP in biological samples. The method utilizes a commercial standard C17-sphingosine 1-phosphate (C17-SPP) derivatized with naphthalene-2,3-dicarboxaldehyde (NDA), a fluorogenic dye used to label primary amines. The detection limit for the C17-SPP NDA was 0.54 fmol. We quantified the C18-SPP in leukemic human cells before and after irradiation by gamma rays. We demonstrated that the amounts of this sphingolipid decreased after the irradiation. In a second part of this work, we used the technique to evaluate the ability of a novel transparent fused-silica capillary, which allows the use of fused-silica capillaries without burning a window. Solarization, homogeneity, and sensitivity were studied using this capillary. The results demonstrate that this new durable capillary can provide a sensitive and reproducible quantitation procedure for CE-LIF studies. | lld:pubmed |
| pubmed-article:17685239 | pubmed:language | eng | lld:pubmed |
| pubmed-article:17685239 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17685239 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:17685239 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17685239 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17685239 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17685239 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17685239 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17685239 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17685239 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17685239 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:17685239 | pubmed:issn | 1946-4940 | lld:pubmed |
| pubmed-article:17685239 | pubmed:author | pubmed-author:UstaJulnarJ | lld:pubmed |
| pubmed-article:17685239 | pubmed:author | pubmed-author:PoinsotVéréna... | lld:pubmed |
| pubmed-article:17685239 | pubmed:author | pubmed-author:CoudercFranço... | lld:pubmed |
| pubmed-article:17685239 | pubmed:author | pubmed-author:RibeiroSidney... | lld:pubmed |
| pubmed-article:17685239 | pubmed:author | pubmed-author:MessaddeqYoun... | lld:pubmed |
| pubmed-article:17685239 | pubmed:author | pubmed-author:RammouzGeorge... | lld:pubmed |
| pubmed-article:17685239 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:17685239 | pubmed:volume | 10 | lld:pubmed |
| pubmed-article:17685239 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:17685239 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:17685239 | pubmed:pagination | 25-31 | lld:pubmed |
| pubmed-article:17685239 | pubmed:dateRevised | 2009-9-11 | lld:pubmed |
| pubmed-article:17685239 | pubmed:meshHeading | pubmed-meshheading:17685239... | lld:pubmed |
| pubmed-article:17685239 | pubmed:meshHeading | pubmed-meshheading:17685239... | lld:pubmed |
| pubmed-article:17685239 | pubmed:meshHeading | pubmed-meshheading:17685239... | lld:pubmed |
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| pubmed-article:17685239 | pubmed:meshHeading | pubmed-meshheading:17685239... | lld:pubmed |
| pubmed-article:17685239 | pubmed:meshHeading | pubmed-meshheading:17685239... | lld:pubmed |
| pubmed-article:17685239 | pubmed:meshHeading | pubmed-meshheading:17685239... | lld:pubmed |
| pubmed-article:17685239 | pubmed:meshHeading | pubmed-meshheading:17685239... | lld:pubmed |
| pubmed-article:17685239 | pubmed:meshHeading | pubmed-meshheading:17685239... | lld:pubmed |
| pubmed-article:17685239 | pubmed:meshHeading | pubmed-meshheading:17685239... | lld:pubmed |
| pubmed-article:17685239 | pubmed:meshHeading | pubmed-meshheading:17685239... | lld:pubmed |
| pubmed-article:17685239 | pubmed:meshHeading | pubmed-meshheading:17685239... | lld:pubmed |
| pubmed-article:17685239 | pubmed:meshHeading | pubmed-meshheading:17685239... | lld:pubmed |
| pubmed-article:17685239 | pubmed:meshHeading | pubmed-meshheading:17685239... | lld:pubmed |
| pubmed-article:17685239 | pubmed:year | 2007 | lld:pubmed |
| pubmed-article:17685239 | pubmed:articleTitle | Analysis of sphingosine 1-phosphate by capillary electrophoresis coupled to laser-induced fluorescence detection: use of a transparent fused-silica capillary. | lld:pubmed |
| pubmed-article:17685239 | pubmed:affiliation | Université Paul Sabatier, IMRCP, Toulouse, France. | lld:pubmed |
| pubmed-article:17685239 | pubmed:publicationType | Journal Article | lld:pubmed |
