| pubmed-article:17645867 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:17645867 | lifeskim:mentions | umls-concept:C0026473 | lld:lifeskim |
| pubmed-article:17645867 | lifeskim:mentions | umls-concept:C0542341 | lld:lifeskim |
| pubmed-article:17645867 | lifeskim:mentions | umls-concept:C0221099 | lld:lifeskim |
| pubmed-article:17645867 | lifeskim:mentions | umls-concept:C0205082 | lld:lifeskim |
| pubmed-article:17645867 | lifeskim:mentions | umls-concept:C0060549 | lld:lifeskim |
| pubmed-article:17645867 | lifeskim:mentions | umls-concept:C0016342 | lld:lifeskim |
| pubmed-article:17645867 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:17645867 | pubmed:dateCreated | 2007-8-7 | lld:pubmed |
| pubmed-article:17645867 | pubmed:abstractText | For rapid chemotaxis quantification, cell prelabelling is often performed with the fluorochrome calcein acetomethylester (calcein AM). We investigated whether calcein AM-prelabelling is reliable for monocyte migration analysis. Human monocytes were either preexposed to calcein AM or unlabelled. Monocyte migration towards the potent chemoattractants transforming growth factor-beta1 (TGF-beta1) and N-formyl-Methionin-Leucin-Phenylalanin (fMLP) was assessed using a 48-well micro-chemotaxis chamber. For quantification, cells were visualized by light microscopy and counted. Surprisingly, random migration of calcein AM-prelabelled cells was significantly impaired compared to the unlabelled control. Accordingly, monocyte chemotaxis towards either TGF-beta1 or fMLP dramatically declined. Adherence of calcein AM-labelled monocytes on plastic was also significantly decreased compared to control cells. As adhesion is regarded as an essential component of monocyte migration, the reduced migration observed in calcein AM-labelled monocytes might be explained by a fluorochrome-induced adhesion defect. Therefore, use of the fluorochrome calcein AM cannot be recommended for functional testing of monocytes. | lld:pubmed |
| pubmed-article:17645867 | pubmed:language | eng | lld:pubmed |
| pubmed-article:17645867 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17645867 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:17645867 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17645867 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17645867 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17645867 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:17645867 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:17645867 | pubmed:issn | 0006-291X | lld:pubmed |
| pubmed-article:17645867 | pubmed:author | pubmed-author:WaltenbergerJ... | lld:pubmed |
| pubmed-article:17645867 | pubmed:author | pubmed-author:CzepluchFrauk... | lld:pubmed |
| pubmed-article:17645867 | pubmed:author | pubmed-author:OlieslagersSe... | lld:pubmed |
| pubmed-article:17645867 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:17645867 | pubmed:day | 21 | lld:pubmed |
| pubmed-article:17645867 | pubmed:volume | 361 | lld:pubmed |
| pubmed-article:17645867 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:17645867 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:17645867 | pubmed:pagination | 410-3 | lld:pubmed |
| pubmed-article:17645867 | pubmed:meshHeading | pubmed-meshheading:17645867... | lld:pubmed |
| pubmed-article:17645867 | pubmed:meshHeading | pubmed-meshheading:17645867... | lld:pubmed |
| pubmed-article:17645867 | pubmed:meshHeading | pubmed-meshheading:17645867... | lld:pubmed |
| pubmed-article:17645867 | pubmed:meshHeading | pubmed-meshheading:17645867... | lld:pubmed |
| pubmed-article:17645867 | pubmed:meshHeading | pubmed-meshheading:17645867... | lld:pubmed |
| pubmed-article:17645867 | pubmed:meshHeading | pubmed-meshheading:17645867... | lld:pubmed |
| pubmed-article:17645867 | pubmed:meshHeading | pubmed-meshheading:17645867... | lld:pubmed |
| pubmed-article:17645867 | pubmed:meshHeading | pubmed-meshheading:17645867... | lld:pubmed |
| pubmed-article:17645867 | pubmed:year | 2007 | lld:pubmed |
| pubmed-article:17645867 | pubmed:articleTitle | Monocyte function is severely impaired by the fluorochrome calcein acetomethylester. | lld:pubmed |
| pubmed-article:17645867 | pubmed:affiliation | Department of Cardiology, Cardiovascular Research Institute Maastricht, University of Maastricht, P. Debyelaan 25, P.O. Box 5800, 6202 AZ Maastricht, Maastricht, The Netherlands. | lld:pubmed |
| pubmed-article:17645867 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17645867 | lld:pubmed |
