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pubmed-article:17628621pubmed:dateCreated2007-10-1lld:pubmed
pubmed-article:17628621pubmed:abstractTextA search was made for conjugates of indole-3-acetic acid (IAA) in rice (Oryza sativa) using liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) in order to elucidate unknown metabolic pathways for IAA. N-beta-d-Glucopyranosyl indole-3-acetic acid (IAA-N-Glc) was found in an alkaline hydrolysate of rice extract. A quantitative analysis of 3-week-old rice demonstrated that the total amount of IAA-N-Glc was equal to that of IAA. A LC-ESI-MS/MS-based analysis established that the major part of IAA-N-Glc was present as bound forms with aspartate and glutamate. Their levels were in good agreement with the total amount of IAA-N-Glc during the vegetative growth of rice. Further detailed analysis showed that both conjugates highly accumulated in the root. The free form of IAA-N-Glc accounted for 60% of the total in seeds but could not be detected in the vegetative tissue. An incorporation study using deuterium-labeled compounds showed that the amino acid conjugates of IAA-N-Glc were biosynthesized from IAA-amino acids. IAA-N-Glc and/or its conjugates were also found in extracts of Arabidopsis, Lotus japonicus, and maize, suggesting that N-glucosylation of indole can be the common metabolic pathway of IAA in plants.lld:pubmed
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pubmed-article:17628621pubmed:authorpubmed-author:KennyM RMRlld:pubmed
pubmed-article:17628621pubmed:authorpubmed-author:MiyagawaHisas...lld:pubmed
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pubmed-article:17628621pubmed:articleTitleMetabolism of indole-3-acetic acid in rice: identification and characterization of N-beta-D-glucopyranosyl indole-3-acetic acid and its conjugates.lld:pubmed
pubmed-article:17628621pubmed:affiliationDivision of Applied Life Science, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.lld:pubmed
pubmed-article:17628621pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:17628621pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed