| pubmed-article:17585299 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:17585299 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:17585299 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:17585299 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
| pubmed-article:17585299 | pubmed:issue | 7 | lld:pubmed |
| pubmed-article:17585299 | pubmed:dateCreated | 2007-6-22 | lld:pubmed |
| pubmed-article:17585299 | pubmed:abstractText | This proteomic protocol purifies and identifies palmitoylated proteins (i.e., S-acylated proteins) from complex protein extracts. The method relies on an acyl-biotinyl exchange chemistry in which biotin moieties are substituted for the thioester-linked protein acyl-modifications through a sequence of three in vitro chemical steps: (i) blockade of free thiols with N-ethylmaleimide; (ii) cleavage of the Cys-palmitoyl thioester linkages with hydroxylamine; and (iii) labeling of thiols, newly exposed by the hydroxylamine, with biotin-HPDP (Biotin-HPDP-N-[6-(Biotinamido)hexyl]-3'-(2'-pyridyldithio)propionamide. The biotinylated proteins are then affinity-purified using streptavidin-agarose and identified by multi-dimensional protein identification technology (MuDPIT), a high-throughput, tandem mass spectrometry (MS/MS)-based proteomic technology. MuDPIT also affords a semi-quantitative analysis that may be used to assess the gross changes induced to the global palmitoylation profile by mutation or drugs. Typically, 2-3 weeks are required for this analysis. | lld:pubmed |
| pubmed-article:17585299 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17585299 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17585299 | pubmed:language | eng | lld:pubmed |
| pubmed-article:17585299 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17585299 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:17585299 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17585299 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17585299 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17585299 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:17585299 | pubmed:issn | 1750-2799 | lld:pubmed |
| pubmed-article:17585299 | pubmed:author | pubmed-author:DavisNicholas... | lld:pubmed |
| pubmed-article:17585299 | pubmed:author | pubmed-author:RothAmy FAF | lld:pubmed |
| pubmed-article:17585299 | pubmed:author | pubmed-author:WanJunmeiJ | lld:pubmed |
| pubmed-article:17585299 | pubmed:author | pubmed-author:BaileyAaron... | lld:pubmed |
| pubmed-article:17585299 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:17585299 | pubmed:volume | 2 | lld:pubmed |
| pubmed-article:17585299 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:17585299 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:17585299 | pubmed:pagination | 1573-84 | lld:pubmed |
| pubmed-article:17585299 | pubmed:dateRevised | 2008-3-24 | lld:pubmed |
| pubmed-article:17585299 | pubmed:meshHeading | pubmed-meshheading:17585299... | lld:pubmed |
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| pubmed-article:17585299 | pubmed:meshHeading | pubmed-meshheading:17585299... | lld:pubmed |
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| pubmed-article:17585299 | pubmed:meshHeading | pubmed-meshheading:17585299... | lld:pubmed |
| pubmed-article:17585299 | pubmed:year | 2007 | lld:pubmed |
| pubmed-article:17585299 | pubmed:articleTitle | Palmitoylated proteins: purification and identification. | lld:pubmed |
| pubmed-article:17585299 | pubmed:affiliation | Department of Pharmacology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA. | lld:pubmed |
| pubmed-article:17585299 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:17585299 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
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