| pubmed-article:17583944 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:17583944 | lifeskim:mentions | umls-concept:C0004927 | lld:lifeskim |
| pubmed-article:17583944 | lifeskim:mentions | umls-concept:C0184511 | lld:lifeskim |
| pubmed-article:17583944 | lifeskim:mentions | umls-concept:C1521991 | lld:lifeskim |
| pubmed-article:17583944 | lifeskim:mentions | umls-concept:C1337615 | lld:lifeskim |
| pubmed-article:17583944 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:17583944 | pubmed:issue | 28 | lld:pubmed |
| pubmed-article:17583944 | pubmed:dateCreated | 2007-7-12 | lld:pubmed |
| pubmed-article:17583944 | pubmed:abstractText | Pairs of fluorophores in close proximity often show self-quenching of fluorescence by the well-known H-dimer mechanism. We use a pair of fluorophores in the new dicyanomethylenedihydrofuran (DCDHF) dye family in the design and characterization of a new fluorescent probe for nucleic acid detection, which we refer to as a self-quenched intramolecular dimer (SQuID) molecular beacon (MB). We obtain a quenching efficiency of 97.2%, higher than the only other reported value for a MB employing fluorophore self-quenching by H-dimer formation. Furthermore, the excellent single-molecule (SM) emitter characteristics of the DCDHF dyes allow observation of individual SQuID MB-target complexes immobilized on a surface, where the doubled SM emission intensity of our target-bound beacon ensures a higher signal-to-background ratio than conventional fluorophore-quencher MBs. Additional advantages of the SQuID MB are single-pot labeling, visible colorimetric detection of the target, and intrinsic single-molecule two-step photobleaching behavior, which offers a specific means of discriminating between functional MBs and spurious fluorescence. | lld:pubmed |
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| pubmed-article:17583944 | pubmed:language | eng | lld:pubmed |
| pubmed-article:17583944 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17583944 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:17583944 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:17583944 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:17583944 | pubmed:issn | 1520-6106 | lld:pubmed |
| pubmed-article:17583944 | pubmed:author | pubmed-author:MoernerW EWE | lld:pubmed |
| pubmed-article:17583944 | pubmed:author | pubmed-author:TwiegRobert... | lld:pubmed |
| pubmed-article:17583944 | pubmed:author | pubmed-author:WangHuiH | lld:pubmed |
| pubmed-article:17583944 | pubmed:author | pubmed-author:ConleyNichola... | lld:pubmed |
| pubmed-article:17583944 | pubmed:author | pubmed-author:PomerantzAndr... | lld:pubmed |
| pubmed-article:17583944 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:17583944 | pubmed:day | 19 | lld:pubmed |
| pubmed-article:17583944 | pubmed:volume | 111 | lld:pubmed |
| pubmed-article:17583944 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:17583944 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:17583944 | pubmed:pagination | 7929-31 | lld:pubmed |
| pubmed-article:17583944 | pubmed:dateRevised | 2011-6-13 | lld:pubmed |
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| pubmed-article:17583944 | pubmed:meshHeading | pubmed-meshheading:17583944... | lld:pubmed |
| pubmed-article:17583944 | pubmed:year | 2007 | lld:pubmed |
| pubmed-article:17583944 | pubmed:articleTitle | Bulk and single-molecule characterization of an improved molecular beacon utilizing H-dimer excitonic behavior. | lld:pubmed |
| pubmed-article:17583944 | pubmed:publicationType | Letter | lld:pubmed |
| pubmed-article:17583944 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:17583944 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:17583944 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
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