| pubmed-article:17569634 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:17569634 | lifeskim:mentions | umls-concept:C0028948 | lld:lifeskim |
| pubmed-article:17569634 | lifeskim:mentions | umls-concept:C0242485 | lld:lifeskim |
| pubmed-article:17569634 | lifeskim:mentions | umls-concept:C0597731 | lld:lifeskim |
| pubmed-article:17569634 | lifeskim:mentions | umls-concept:C0936012 | lld:lifeskim |
| pubmed-article:17569634 | pubmed:dateCreated | 2007-6-15 | lld:pubmed |
| pubmed-article:17569634 | pubmed:abstractText | A flow injection SPR spectrometer is used to detect DNA hybridization via amplification by an enzyme-catalyzed precipitation reaction. Hybridization of an oligodeoxynucleotide (ODN) target with a surface-confined ODN probe, followed by hybridization between the overhang on the resultant duplex and a biotin-tagged ODN, yields a "sandwich" complex (a three-component double-stranded). A streptavidin-horseradish peroxidase conjugate (SA-HRP) can be attached to the sandwich complex-covered surface via the biotin/streptavidin complexation. In the presence of H2O2, the HRP catalyzes oxidation of 4-chloro-1-naphthol (CN) to form a precipitate on the sensor surface. The precipitated film dramatically changes the refractive index at the metal/dielectric interface and significantly lowers the detection level. The method is shown to be reproducible, to possess high ODN sequence specificity and a high sensitivity allowing detection of ODN target concentration as low as 10 fM. | lld:pubmed |
| pubmed-article:17569634 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17569634 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17569634 | pubmed:language | eng | lld:pubmed |
| pubmed-article:17569634 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17569634 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:17569634 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17569634 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:17569634 | pubmed:issn | 1093-4715 | lld:pubmed |
| pubmed-article:17569634 | pubmed:author | pubmed-author:ZhangYiY | lld:pubmed |
| pubmed-article:17569634 | pubmed:author | pubmed-author:WangJianxiuJ | lld:pubmed |
| pubmed-article:17569634 | pubmed:author | pubmed-author:ZhouFeimengF | lld:pubmed |
| pubmed-article:17569634 | pubmed:author | pubmed-author:ZengDongmingD | lld:pubmed |
| pubmed-article:17569634 | pubmed:author | pubmed-author:YinLijunL | lld:pubmed |
| pubmed-article:17569634 | pubmed:author | pubmed-author:ZhangYintangY | lld:pubmed |
| pubmed-article:17569634 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:17569634 | pubmed:volume | 12 | lld:pubmed |
| pubmed-article:17569634 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:17569634 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:17569634 | pubmed:pagination | 5117-23 | lld:pubmed |
| pubmed-article:17569634 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
| pubmed-article:17569634 | pubmed:meshHeading | pubmed-meshheading:17569634... | lld:pubmed |
| pubmed-article:17569634 | pubmed:meshHeading | pubmed-meshheading:17569634... | lld:pubmed |
| pubmed-article:17569634 | pubmed:meshHeading | pubmed-meshheading:17569634... | lld:pubmed |
| pubmed-article:17569634 | pubmed:meshHeading | pubmed-meshheading:17569634... | lld:pubmed |
| pubmed-article:17569634 | pubmed:meshHeading | pubmed-meshheading:17569634... | lld:pubmed |
| pubmed-article:17569634 | pubmed:meshHeading | pubmed-meshheading:17569634... | lld:pubmed |
| pubmed-article:17569634 | pubmed:year | 2007 | lld:pubmed |
| pubmed-article:17569634 | pubmed:articleTitle | Sequence-specific analysis of oligodeoxynucleotides by precipitate-amplified surface plasmon resonance measurements. | lld:pubmed |
| pubmed-article:17569634 | pubmed:affiliation | College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan, PR China. | lld:pubmed |
| pubmed-article:17569634 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:17569634 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:17569634 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:17569634 | pubmed:publicationType | Evaluation Studies | lld:pubmed |
| pubmed-article:17569634 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
