| pubmed-article:17555338 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:17555338 | lifeskim:mentions | umls-concept:C0001694 | lld:lifeskim |
| pubmed-article:17555338 | lifeskim:mentions | umls-concept:C0038884 | lld:lifeskim |
| pubmed-article:17555338 | lifeskim:mentions | umls-concept:C0086296 | lld:lifeskim |
| pubmed-article:17555338 | lifeskim:mentions | umls-concept:C2603343 | lld:lifeskim |
| pubmed-article:17555338 | lifeskim:mentions | umls-concept:C1704608 | lld:lifeskim |
| pubmed-article:17555338 | lifeskim:mentions | umls-concept:C0872351 | lld:lifeskim |
| pubmed-article:17555338 | lifeskim:mentions | umls-concept:C0022381 | lld:lifeskim |
| pubmed-article:17555338 | pubmed:issue | 14 | lld:pubmed |
| pubmed-article:17555338 | pubmed:dateCreated | 2007-6-26 | lld:pubmed |
| pubmed-article:17555338 | pubmed:abstractText | In this paper, we studied the surface properties of recombinant aequorin at the air-water interface. Using the Langmuir monolayer technique, the surface properties of aequorin were studied, including the surface pressure and surface potential-area isotherms, compression-decompression cycles, and stability on Trizma Base (Tris/HCl) buffer at pH 7.6. The results showed that aequorin formed a stable Langmuir monolayer and the surface pressure-area isotherms were dependent on both pH and ionic strength. At a pH higher or lower than 7.6, the limiting molecular area decreased. The circular dichroism (CD) spectra of aequorin in aqueous solutions explained this result: when the pH was higher than 7.6, the alpha-helix conformation changed to unordered structures, whereas at a pH lower than 7.6, the alpha-helix conformation changed to beta-sheet. The addition of calcium chloride to the Tris/HCl buffer subphase (pH 7.6) caused an increase of the limiting molecular area of the aequorin Langmuir monolayer. The fluorescence spectra of a Langmuir-Blodgett (LB) film of aequorin in the presence of calcium chloride indicated that the aequorin transformed to the apoaequorin. | lld:pubmed |
| pubmed-article:17555338 | pubmed:language | eng | lld:pubmed |
| pubmed-article:17555338 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17555338 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:17555338 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17555338 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17555338 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17555338 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17555338 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17555338 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17555338 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17555338 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17555338 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17555338 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:17555338 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:17555338 | pubmed:issn | 0743-7463 | lld:pubmed |
| pubmed-article:17555338 | pubmed:author | pubmed-author:DaunertSylvia... | lld:pubmed |
| pubmed-article:17555338 | pubmed:author | pubmed-author:LeblancRoger... | lld:pubmed |
| pubmed-article:17555338 | pubmed:author | pubmed-author:MicicMiodragM | lld:pubmed |
| pubmed-article:17555338 | pubmed:author | pubmed-author:WangChengshan... | lld:pubmed |
| pubmed-article:17555338 | pubmed:author | pubmed-author:EnsorMarkM | lld:pubmed |
| pubmed-article:17555338 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:17555338 | pubmed:day | 3 | lld:pubmed |
| pubmed-article:17555338 | pubmed:volume | 23 | lld:pubmed |
| pubmed-article:17555338 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:17555338 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:17555338 | pubmed:pagination | 7602-7 | lld:pubmed |
| pubmed-article:17555338 | pubmed:meshHeading | pubmed-meshheading:17555338... | lld:pubmed |
| pubmed-article:17555338 | pubmed:meshHeading | pubmed-meshheading:17555338... | lld:pubmed |
| pubmed-article:17555338 | pubmed:meshHeading | pubmed-meshheading:17555338... | lld:pubmed |
| pubmed-article:17555338 | pubmed:meshHeading | pubmed-meshheading:17555338... | lld:pubmed |
| pubmed-article:17555338 | pubmed:meshHeading | pubmed-meshheading:17555338... | lld:pubmed |
| pubmed-article:17555338 | pubmed:meshHeading | pubmed-meshheading:17555338... | lld:pubmed |
| pubmed-article:17555338 | pubmed:meshHeading | pubmed-meshheading:17555338... | lld:pubmed |
| pubmed-article:17555338 | pubmed:meshHeading | pubmed-meshheading:17555338... | lld:pubmed |
| pubmed-article:17555338 | pubmed:meshHeading | pubmed-meshheading:17555338... | lld:pubmed |
| pubmed-article:17555338 | pubmed:meshHeading | pubmed-meshheading:17555338... | lld:pubmed |
| pubmed-article:17555338 | pubmed:meshHeading | pubmed-meshheading:17555338... | lld:pubmed |
| pubmed-article:17555338 | pubmed:meshHeading | pubmed-meshheading:17555338... | lld:pubmed |
| pubmed-article:17555338 | pubmed:meshHeading | pubmed-meshheading:17555338... | lld:pubmed |
| pubmed-article:17555338 | pubmed:meshHeading | pubmed-meshheading:17555338... | lld:pubmed |
| pubmed-article:17555338 | pubmed:year | 2007 | lld:pubmed |
| pubmed-article:17555338 | pubmed:articleTitle | Surface properties of "jellyfish": Langmuir monolayer and Langmuir-Blodgett film studies of recombinant aequorin. | lld:pubmed |
| pubmed-article:17555338 | pubmed:affiliation | Department of Chemistry, University of Miami, Coral Gables, Florida 33146, USA. | lld:pubmed |
| pubmed-article:17555338 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:17555338 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:17555338 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
