| pubmed-article:17550589 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:17550589 | lifeskim:mentions | umls-concept:C0021344 | lld:lifeskim |
| pubmed-article:17550589 | lifeskim:mentions | umls-concept:C0162326 | lld:lifeskim |
| pubmed-article:17550589 | lifeskim:mentions | umls-concept:C0917792 | lld:lifeskim |
| pubmed-article:17550589 | lifeskim:mentions | umls-concept:C1285573 | lld:lifeskim |
| pubmed-article:17550589 | lifeskim:mentions | umls-concept:C0020003 | lld:lifeskim |
| pubmed-article:17550589 | lifeskim:mentions | umls-concept:C0022879 | lld:lifeskim |
| pubmed-article:17550589 | lifeskim:mentions | umls-concept:C0205547 | lld:lifeskim |
| pubmed-article:17550589 | pubmed:dateCreated | 2007-6-22 | lld:pubmed |
| pubmed-article:17550589 | pubmed:abstractText | Human papillomavirus (HPV) genotyping is important for following up patients with persistent HPV infection and for evaluation of prevention strategy for the individual patients to be immunized with type-specific HPV vaccines. The aim of this study was to optimize a robust "low-temperature" (LoTemp) PCR system to streamline the research protocols for HPV DNA nested PCR-amplification followed by genotyping with direct DNA sequencing. The protocol optimization facilitates transferring this molecular technology into clinical laboratory practice. In particular, lowering the temperature by 10 degrees C at each step of thermocycling during in vitro DNA amplification yields more homogeneous PCR products. With this protocol, template purification before enzymatic cycle primer extensions is no longer necessary. | lld:pubmed |
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| pubmed-article:17550589 | pubmed:language | eng | lld:pubmed |
| pubmed-article:17550589 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17550589 | pubmed:status | PubMed-not-MEDLINE | lld:pubmed |
| pubmed-article:17550589 | pubmed:issn | 1750-9378 | lld:pubmed |
| pubmed-article:17550589 | pubmed:author | pubmed-author:LeeSin HangSH | lld:pubmed |
| pubmed-article:17550589 | pubmed:author | pubmed-author:VigliottiVero... | lld:pubmed |
| pubmed-article:17550589 | pubmed:author | pubmed-author:VigliottiJess... | lld:pubmed |
| pubmed-article:17550589 | pubmed:author | pubmed-author:PappuSuriS | lld:pubmed |
| pubmed-article:17550589 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:17550589 | pubmed:volume | 2 | lld:pubmed |
| pubmed-article:17550589 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:17550589 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:17550589 | pubmed:pagination | 11 | lld:pubmed |
| pubmed-article:17550589 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:17550589 | pubmed:year | 2007 | lld:pubmed |
| pubmed-article:17550589 | pubmed:articleTitle | Routine human papillomavirus genotyping by DNA sequencing in community hospital laboratories. | lld:pubmed |
| pubmed-article:17550589 | pubmed:affiliation | Department of Pathology, Milford Hospital, Milford, Connecticut, USA. sinhang.lee@milfordhospital.org | lld:pubmed |
| pubmed-article:17550589 | pubmed:publicationType | Journal Article | lld:pubmed |
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