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pubmed-article:17522381pubmed:abstractTextLipopolysaccharide (LPS), a bacterial endotoxin, triggers deleterious systemic inflammatory responses when released into blood circulation, causing organ dysfunction and death. In response to LPS stimulation, CD14 and toll-like receptor (TLR)-4 elicit inflammatory signaling cascades. Although leukocyte integrins (CD11b/CD18 and CD11c/CD18) were reported to bind LPS and induce NF-kappaB translocation, the evidence on such epitope location remains elusive. The present study aims to delineate the LPS-binding sites on the integrin CD18 antigen and to design peptide(s) as potential prophylactic and/or therapeutic agents to modulate LPS effects in activated Jurkat cells. Epitope mapping analysis using a series of CD18 truncated variants revealed two putative LPS-binding sites within the betaA region (216-248 and 266-318 a.a.), which were further confirmed by point mutation studies. Inhibition assay demonstrated that the CD18-betaA(266-318) peptide could block LPS binding in a dose-dependent manner. Our data also indicated that treatment with the CD18-peptide modulated TNF-alpha mRNA transcription via the NF-kappaB signaling pathway in LPS-activated Jurkat cells. In conclusion, we have identified two novel LPS-binding sites located at the CD18 betaA domain of leukocyte integrin, and the integrin peptide betaA(266-318) is shown to inhibit LPS binding and subsequent inflammatory events, having therapeutic implications to cure gram-negative endotoxemia.lld:pubmed
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pubmed-article:17522381pubmed:articleTitleCharacterization of two novel LPS-binding sites in leukocyte integrin betaA domain.lld:pubmed
pubmed-article:17522381pubmed:affiliationDepartment of Surgery, The University of Hong Kong, Jockey Club Clinical Research Center, 21 Sassoon Rd., Pokfulam, Hong Kong.lld:pubmed
pubmed-article:17522381pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:17522381pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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