pubmed-article:17522381 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:17522381 | lifeskim:mentions | umls-concept:C0023516 | lld:lifeskim |
pubmed-article:17522381 | lifeskim:mentions | umls-concept:C0205145 | lld:lifeskim |
pubmed-article:17522381 | lifeskim:mentions | umls-concept:C0021701 | lld:lifeskim |
pubmed-article:17522381 | lifeskim:mentions | umls-concept:C1514562 | lld:lifeskim |
pubmed-article:17522381 | lifeskim:mentions | umls-concept:C1883221 | lld:lifeskim |
pubmed-article:17522381 | lifeskim:mentions | umls-concept:C0679622 | lld:lifeskim |
pubmed-article:17522381 | lifeskim:mentions | umls-concept:C1883204 | lld:lifeskim |
pubmed-article:17522381 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
pubmed-article:17522381 | lifeskim:mentions | umls-concept:C0205314 | lld:lifeskim |
pubmed-article:17522381 | lifeskim:mentions | umls-concept:C1880389 | lld:lifeskim |
pubmed-article:17522381 | pubmed:issue | 12 | lld:pubmed |
pubmed-article:17522381 | pubmed:dateCreated | 2007-10-1 | lld:pubmed |
pubmed-article:17522381 | pubmed:abstractText | Lipopolysaccharide (LPS), a bacterial endotoxin, triggers deleterious systemic inflammatory responses when released into blood circulation, causing organ dysfunction and death. In response to LPS stimulation, CD14 and toll-like receptor (TLR)-4 elicit inflammatory signaling cascades. Although leukocyte integrins (CD11b/CD18 and CD11c/CD18) were reported to bind LPS and induce NF-kappaB translocation, the evidence on such epitope location remains elusive. The present study aims to delineate the LPS-binding sites on the integrin CD18 antigen and to design peptide(s) as potential prophylactic and/or therapeutic agents to modulate LPS effects in activated Jurkat cells. Epitope mapping analysis using a series of CD18 truncated variants revealed two putative LPS-binding sites within the betaA region (216-248 and 266-318 a.a.), which were further confirmed by point mutation studies. Inhibition assay demonstrated that the CD18-betaA(266-318) peptide could block LPS binding in a dose-dependent manner. Our data also indicated that treatment with the CD18-peptide modulated TNF-alpha mRNA transcription via the NF-kappaB signaling pathway in LPS-activated Jurkat cells. In conclusion, we have identified two novel LPS-binding sites located at the CD18 betaA domain of leukocyte integrin, and the integrin peptide betaA(266-318) is shown to inhibit LPS binding and subsequent inflammatory events, having therapeutic implications to cure gram-negative endotoxemia. | lld:pubmed |
pubmed-article:17522381 | pubmed:language | eng | lld:pubmed |
pubmed-article:17522381 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:17522381 | pubmed:citationSubset | IM | lld:pubmed |
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pubmed-article:17522381 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:17522381 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:17522381 | pubmed:month | Oct | lld:pubmed |
pubmed-article:17522381 | pubmed:issn | 1530-6860 | lld:pubmed |
pubmed-article:17522381 | pubmed:author | pubmed-author:FanSheung-Tat... | lld:pubmed |
pubmed-article:17522381 | pubmed:author | pubmed-author:KlicksteinLlo... | lld:pubmed |
pubmed-article:17522381 | pubmed:author | pubmed-author:ChengR... | lld:pubmed |
pubmed-article:17522381 | pubmed:author | pubmed-author:LukJohn MJM | lld:pubmed |
pubmed-article:17522381 | pubmed:author | pubmed-author:WongKwong-Fai... | lld:pubmed |
pubmed-article:17522381 | pubmed:issnType | Electronic | lld:pubmed |
pubmed-article:17522381 | pubmed:volume | 21 | lld:pubmed |
pubmed-article:17522381 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:17522381 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:17522381 | pubmed:pagination | 3231-9 | lld:pubmed |
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pubmed-article:17522381 | pubmed:meshHeading | pubmed-meshheading:17522381... | lld:pubmed |
pubmed-article:17522381 | pubmed:year | 2007 | lld:pubmed |
pubmed-article:17522381 | pubmed:articleTitle | Characterization of two novel LPS-binding sites in leukocyte integrin betaA domain. | lld:pubmed |
pubmed-article:17522381 | pubmed:affiliation | Department of Surgery, The University of Hong Kong, Jockey Club Clinical Research Center, 21 Sassoon Rd., Pokfulam, Hong Kong. | lld:pubmed |
pubmed-article:17522381 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:17522381 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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