| pubmed-article:17516243 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:17516243 | lifeskim:mentions | umls-concept:C0521119 | lld:lifeskim |
| pubmed-article:17516243 | lifeskim:mentions | umls-concept:C0178719 | lld:lifeskim |
| pubmed-article:17516243 | lifeskim:mentions | umls-concept:C0038836 | lld:lifeskim |
| pubmed-article:17516243 | lifeskim:mentions | umls-concept:C0068355 | lld:lifeskim |
| pubmed-article:17516243 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:17516243 | lifeskim:mentions | umls-concept:C0016315 | lld:lifeskim |
| pubmed-article:17516243 | lifeskim:mentions | umls-concept:C0936012 | lld:lifeskim |
| pubmed-article:17516243 | lifeskim:mentions | umls-concept:C0644810 | lld:lifeskim |
| pubmed-article:17516243 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:17516243 | pubmed:dateCreated | 2007-5-22 | lld:pubmed |
| pubmed-article:17516243 | pubmed:abstractText | All methods used for quantitation of superoxide have limitations when it comes to differentiating between extracellular and intracellular sites of superoxide production. In the present study, we monitored dihydroethidium (DHE)-derived fluorescence at 570 nm, which indicates hydroxyethidium derived from reaction with superoxide produced by human leukemia cells (HL-60) and microvascular endothelial cells (HMEC-1). Phorbol-12-myristate 13-acetate (PMA; 100 ng/ml) caused an increase in fluorescence and lucigenin chemiluminescence in HL-60, which was abolished by superoxide dismutase (SOD; 600 U/ml) indicating that DHE detects extracellular superoxide. Furthermore, both HL-60 cells and HMEC-1 generated a fluorescence signal in the presence of DHE under resting conditions, which was unaffected by SOD, but abolished by polyethylene glycosylated-SOD (PEG-SOD) (100 U/ml) and MnTmPyP (25 microM), indicating that DHE also detects superoxide produced intracellularly. In HMEC-1, silencing of either Nox2 or Nox4 components of NADPH oxidase with small interference RNA (siRNA) resulted in a significant reduction in superoxide detected by both DHE fluorescence (Nox2 siRNA; 71 +/- 6% and Nox4 siRNA 83 +/- 7% of control) and lucigenin chemiluminescence (Nox2; 54 +/- 6% and Nox4 74 +/- 4% of control). In conclusion, DHE-derived fluorescence at 570 nm is a convenient method for detection of intracellular and extracellular superoxide produced by phagocytic and vascular NADPH oxidase. | lld:pubmed |
| pubmed-article:17516243 | pubmed:language | eng | lld:pubmed |
| pubmed-article:17516243 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17516243 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:17516243 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17516243 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17516243 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17516243 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17516243 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17516243 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17516243 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17516243 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17516243 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17516243 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17516243 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17516243 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17516243 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17516243 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17516243 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:17516243 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:17516243 | pubmed:issn | 1071-5762 | lld:pubmed |
| pubmed-article:17516243 | pubmed:author | pubmed-author:SelemidisStav... | lld:pubmed |
| pubmed-article:17516243 | pubmed:author | pubmed-author:PeshavariyaHi... | lld:pubmed |
| pubmed-article:17516243 | pubmed:author | pubmed-author:DustingGregor... | lld:pubmed |
| pubmed-article:17516243 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:17516243 | pubmed:volume | 41 | lld:pubmed |
| pubmed-article:17516243 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:17516243 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:17516243 | pubmed:pagination | 699-712 | lld:pubmed |
| pubmed-article:17516243 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
| pubmed-article:17516243 | pubmed:meshHeading | pubmed-meshheading:17516243... | lld:pubmed |
| pubmed-article:17516243 | pubmed:meshHeading | pubmed-meshheading:17516243... | lld:pubmed |
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| pubmed-article:17516243 | pubmed:meshHeading | pubmed-meshheading:17516243... | lld:pubmed |
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| pubmed-article:17516243 | pubmed:meshHeading | pubmed-meshheading:17516243... | lld:pubmed |
| pubmed-article:17516243 | pubmed:meshHeading | pubmed-meshheading:17516243... | lld:pubmed |
| pubmed-article:17516243 | pubmed:meshHeading | pubmed-meshheading:17516243... | lld:pubmed |
| pubmed-article:17516243 | pubmed:meshHeading | pubmed-meshheading:17516243... | lld:pubmed |
| pubmed-article:17516243 | pubmed:meshHeading | pubmed-meshheading:17516243... | lld:pubmed |
| pubmed-article:17516243 | pubmed:year | 2007 | lld:pubmed |
| pubmed-article:17516243 | pubmed:articleTitle | Analysis of dihydroethidium fluorescence for the detection of intracellular and extracellular superoxide produced by NADPH oxidase. | lld:pubmed |
| pubmed-article:17516243 | pubmed:affiliation | Cytoprotection Pharmacology Laboratory, Bernard O'Brien Institute of Microsurgery, University of Melbourne, Melbourne, Vic., Australia. | lld:pubmed |
| pubmed-article:17516243 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:17516243 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17516243 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17516243 | lld:pubmed |
