| pubmed-article:1747375 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1747375 | lifeskim:mentions | umls-concept:C0007600 | lld:lifeskim |
| pubmed-article:1747375 | lifeskim:mentions | umls-concept:C0330390 | lld:lifeskim |
| pubmed-article:1747375 | lifeskim:mentions | umls-concept:C0030281 | lld:lifeskim |
| pubmed-article:1747375 | lifeskim:mentions | umls-concept:C0025519 | lld:lifeskim |
| pubmed-article:1747375 | lifeskim:mentions | umls-concept:C0005528 | lld:lifeskim |
| pubmed-article:1747375 | lifeskim:mentions | umls-concept:C0017725 | lld:lifeskim |
| pubmed-article:1747375 | lifeskim:mentions | umls-concept:C1413028 | lld:lifeskim |
| pubmed-article:1747375 | pubmed:issue | 49 | lld:pubmed |
| pubmed-article:1747375 | pubmed:dateCreated | 1992-1-17 | lld:pubmed |
| pubmed-article:1747375 | pubmed:abstractText | Kinetic characteristics of glucose transport and glucose phosphorylation were studied in the islet cell line beta TC-1 to explore the roles of these processes in determining the dependence of glucose metabolism and insulin secretion on external glucose. The predominant glucose transporter present was the rat brain/erythrocyte type (Glut1), as determined by RNA and immunoblot analysis. The liver/islet glucose transporter (Glut2) RNA was not detected. The functional parameters of zero-trans glucose entry were Km = 9.5 +/- 2 mM and Vmax = 15.2 +/- 2 nmol min-1 (microL of cell water)-1. Phosphorylation kinetics of two hexokinase activities were characterized in situ. A low-Km (0.036 mM) hexokinase with a Vmax of 0.40 nmol min-1 (microL of cell water)-1 was present along with a high-Km (10 mM) hexokinase, which appeared to conform to a cooperative model with a Hill coefficient of about 1.4 and a Vmax of 0.3 nmol min-1 (microL of cell water)-1. Intracellular glucose at steady state was about 80% of the extracellular glucose from 3 to 15 mM, and transport did not limit metabolism in this range. In this static (nonperifusion) system, 2-3 times more immunoreactive insulin was secreted into the medium at 15 mM glucose than at 3 mM. The dependence of insulin secretion on external glucose roughly paralleled the dependence of glucose metabolism on external glucose. Simulations with a model demonstrated the degree to which changes in transport activity would affect intracellular glucose levels and the rate of the high-Km hexokinase (with the potential to affect insulin release). | lld:pubmed |
| pubmed-article:1747375 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1747375 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1747375 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1747375 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1747375 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1747375 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1747375 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:1747375 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1747375 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1747375 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:1747375 | pubmed:issn | 0006-2960 | lld:pubmed |
| pubmed-article:1747375 | pubmed:author | pubmed-author:RegenD MDM | lld:pubmed |
| pubmed-article:1747375 | pubmed:author | pubmed-author:WhitesellR... | lld:pubmed |
| pubmed-article:1747375 | pubmed:author | pubmed-author:AbumradN ANA | lld:pubmed |
| pubmed-article:1747375 | pubmed:author | pubmed-author:PowersA CAC | lld:pubmed |
| pubmed-article:1747375 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1747375 | pubmed:day | 10 | lld:pubmed |
| pubmed-article:1747375 | pubmed:volume | 30 | lld:pubmed |
| pubmed-article:1747375 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1747375 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1747375 | pubmed:pagination | 11560-6 | lld:pubmed |
| pubmed-article:1747375 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
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| pubmed-article:1747375 | pubmed:meshHeading | pubmed-meshheading:1747375-... | lld:pubmed |
| pubmed-article:1747375 | pubmed:year | 1991 | lld:pubmed |
| pubmed-article:1747375 | pubmed:articleTitle | Transport and metabolism of glucose in an insulin-secreting cell line, beta TC-1. | lld:pubmed |
| pubmed-article:1747375 | pubmed:affiliation | Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232. | lld:pubmed |
| pubmed-article:1747375 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1747375 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:1747375 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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