| pubmed-article:17467719 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:17467719 | lifeskim:mentions | umls-concept:C0017953 | lld:lifeskim |
| pubmed-article:17467719 | lifeskim:mentions | umls-concept:C0028959 | lld:lifeskim |
| pubmed-article:17467719 | lifeskim:mentions | umls-concept:C0680730 | lld:lifeskim |
| pubmed-article:17467719 | lifeskim:mentions | umls-concept:C0205554 | lld:lifeskim |
| pubmed-article:17467719 | lifeskim:mentions | umls-concept:C0037813 | lld:lifeskim |
| pubmed-article:17467719 | lifeskim:mentions | umls-concept:C0016315 | lld:lifeskim |
| pubmed-article:17467719 | lifeskim:mentions | umls-concept:C1548779 | lld:lifeskim |
| pubmed-article:17467719 | lifeskim:mentions | umls-concept:C1947902 | lld:lifeskim |
| pubmed-article:17467719 | lifeskim:mentions | umls-concept:C1516801 | lld:lifeskim |
| pubmed-article:17467719 | lifeskim:mentions | umls-concept:C1148554 | lld:lifeskim |
| pubmed-article:17467719 | lifeskim:mentions | umls-concept:C0008565 | lld:lifeskim |
| pubmed-article:17467719 | lifeskim:mentions | umls-concept:C2827499 | lld:lifeskim |
| pubmed-article:17467719 | lifeskim:mentions | umls-concept:C2348791 | lld:lifeskim |
| pubmed-article:17467719 | lifeskim:mentions | umls-concept:C1167624 | lld:lifeskim |
| pubmed-article:17467719 | pubmed:issue | 1-2 | lld:pubmed |
| pubmed-article:17467719 | pubmed:dateCreated | 2007-7-30 | lld:pubmed |
| pubmed-article:17467719 | pubmed:abstractText | Two-dimensional high-performance liquid chromatography using an electrospray ionization time-of-flight mass spectrometry (2D-HPLC-ESI-TOF-MS) system was established for the on-line determination of asparagine-linked oligosaccharides in glycopeptides. The analysis of the oligosaccharides started with the enzymic transglycosylation reaction utilizing Endo-beta-N-acetylglucosaminidase (Endo-M). The oligosaccharides were transferred to a fluorescent acceptor (NDA-Asn-GlcNAc) with Endo-M to produce the fluorescent oligosaccharides. The resulting fluorescent oligosaccharides were specifically isolated from the non-fluorescent oligosaccharides with fluorescence detection after separation by the 1st dimension Amide-80 column. The fraction of fluorescent oligosaccharides was effectively trapped in the anion exchange column. The trapped oligosaccharides were then separated by the 2nd dimension ODS column and sensitively determined by ESI-TOF-MS. Disialo-Asn (a model oligosaccharide) and several oligosaccharides liberated from ovalbumin could be efficiently separated by the 2D-HPLC and identified from the ESI-TOF-MS. Based on these results, the proposed 2D-HPLC-ESI-TOF-MS system may be useful for on-line oligosaccharide analyses. Although the analytical run time is still long, a high-throughput determination will be performed by optimization of the 2D-HPLC conditions. | lld:pubmed |
| pubmed-article:17467719 | pubmed:language | eng | lld:pubmed |
| pubmed-article:17467719 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17467719 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:17467719 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17467719 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17467719 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17467719 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17467719 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17467719 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:17467719 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:17467719 | pubmed:issn | 0021-9673 | lld:pubmed |
| pubmed-article:17467719 | pubmed:author | pubmed-author:Toyo'okaToshi... | lld:pubmed |
| pubmed-article:17467719 | pubmed:author | pubmed-author:FukushimaTake... | lld:pubmed |
| pubmed-article:17467719 | pubmed:author | pubmed-author:InagakiShinsu... | lld:pubmed |
| pubmed-article:17467719 | pubmed:author | pubmed-author:MinJun ZheJZ | lld:pubmed |
| pubmed-article:17467719 | pubmed:author | pubmed-author:KuriharaTakam... | lld:pubmed |
| pubmed-article:17467719 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:17467719 | pubmed:day | 10 | lld:pubmed |
| pubmed-article:17467719 | pubmed:volume | 1160 | lld:pubmed |
| pubmed-article:17467719 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:17467719 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:17467719 | pubmed:pagination | 120-7 | lld:pubmed |
| pubmed-article:17467719 | pubmed:dateRevised | 2009-1-15 | lld:pubmed |
| pubmed-article:17467719 | pubmed:meshHeading | pubmed-meshheading:17467719... | lld:pubmed |
| pubmed-article:17467719 | pubmed:meshHeading | pubmed-meshheading:17467719... | lld:pubmed |
| pubmed-article:17467719 | pubmed:meshHeading | pubmed-meshheading:17467719... | lld:pubmed |
| pubmed-article:17467719 | pubmed:meshHeading | pubmed-meshheading:17467719... | lld:pubmed |
| pubmed-article:17467719 | pubmed:meshHeading | pubmed-meshheading:17467719... | lld:pubmed |
| pubmed-article:17467719 | pubmed:meshHeading | pubmed-meshheading:17467719... | lld:pubmed |
| pubmed-article:17467719 | pubmed:meshHeading | pubmed-meshheading:17467719... | lld:pubmed |
| pubmed-article:17467719 | pubmed:meshHeading | pubmed-meshheading:17467719... | lld:pubmed |
| pubmed-article:17467719 | pubmed:meshHeading | pubmed-meshheading:17467719... | lld:pubmed |
| pubmed-article:17467719 | pubmed:meshHeading | pubmed-meshheading:17467719... | lld:pubmed |
| pubmed-article:17467719 | pubmed:year | 2007 | lld:pubmed |
| pubmed-article:17467719 | pubmed:articleTitle | Fully automated two-dimensional high-performance liquid chromatography with electrospray ionization time-of-flight mass spectrometry for the determination of oligosaccharides in glycopeptides after enzymic fluorescence labeling. | lld:pubmed |
| pubmed-article:17467719 | pubmed:affiliation | Division of Bio-Analytical Chemistry, School of Pharmaceutical Sciences, and COE Program in the 21st Century, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, Japan. | lld:pubmed |
| pubmed-article:17467719 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:17467719 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
