| pubmed-article:17428094 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:17428094 | lifeskim:mentions | umls-concept:C0205208 | lld:lifeskim |
| pubmed-article:17428094 | lifeskim:mentions | umls-concept:C0220781 | lld:lifeskim |
| pubmed-article:17428094 | lifeskim:mentions | umls-concept:C0023688 | lld:lifeskim |
| pubmed-article:17428094 | lifeskim:mentions | umls-concept:C1167622 | lld:lifeskim |
| pubmed-article:17428094 | lifeskim:mentions | umls-concept:C1883254 | lld:lifeskim |
| pubmed-article:17428094 | lifeskim:mentions | umls-concept:C1317973 | lld:lifeskim |
| pubmed-article:17428094 | lifeskim:mentions | umls-concept:C0183683 | lld:lifeskim |
| pubmed-article:17428094 | lifeskim:mentions | umls-concept:C0344211 | lld:lifeskim |
| pubmed-article:17428094 | lifeskim:mentions | umls-concept:C1521721 | lld:lifeskim |
| pubmed-article:17428094 | lifeskim:mentions | umls-concept:C2603343 | lld:lifeskim |
| pubmed-article:17428094 | lifeskim:mentions | umls-concept:C1171411 | lld:lifeskim |
| pubmed-article:17428094 | pubmed:issue | 10 | lld:pubmed |
| pubmed-article:17428094 | pubmed:dateCreated | 2007-5-4 | lld:pubmed |
| pubmed-article:17428094 | pubmed:abstractText | Aminopyrazole derivatives constitute the first class of nonpeptidic rationally designed beta-sheet ligands. Here we describe a double solid-phase protocol for both synthesis and affinity testing. The presented solid-phase synthesis of four types of hybrid compounds relies on the Fmoc strategy and circumvents subsequent HPLC purification by precipitating the final product from organic solution in pure form. Hexa- and octapeptide pendants with internal di- and tetrapeptide bridges are now amenable in high yields to combinatorial synthesis of compound libraries for high-throughput screening purposes. Solid-phase peptide synthesis (SPPS) on an acid-resistant PAM allows us, after PMB deprotection, to subject the free aminopyrazole binding sites in an immobilized state to on-bead assays with fluorescence-labeled peptides. From the fluorescence emission intensity decrease, individual binding constants can be calculated via reference curves by simple application of the law of mass action. Gratifyingly, host/guest complexation can be monitored quantitatively even for those ligands, which are almost insoluble in water. | lld:pubmed |
| pubmed-article:17428094 | pubmed:language | eng | lld:pubmed |
| pubmed-article:17428094 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17428094 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:17428094 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17428094 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17428094 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17428094 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17428094 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17428094 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17428094 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:17428094 | pubmed:month | May | lld:pubmed |
| pubmed-article:17428094 | pubmed:issn | 0022-3263 | lld:pubmed |
| pubmed-article:17428094 | pubmed:author | pubmed-author:SchraderThoma... | lld:pubmed |
| pubmed-article:17428094 | pubmed:author | pubmed-author:RzepeckiPetra... | lld:pubmed |
| pubmed-article:17428094 | pubmed:author | pubmed-author:GeibNinaN | lld:pubmed |
| pubmed-article:17428094 | pubmed:author | pubmed-author:PeiferManuelM | lld:pubmed |
| pubmed-article:17428094 | pubmed:author | pubmed-author:BiesemeierFra... | lld:pubmed |
| pubmed-article:17428094 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:17428094 | pubmed:day | 11 | lld:pubmed |
| pubmed-article:17428094 | pubmed:volume | 72 | lld:pubmed |
| pubmed-article:17428094 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:17428094 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:17428094 | pubmed:pagination | 3614-24 | lld:pubmed |
| pubmed-article:17428094 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
| pubmed-article:17428094 | pubmed:meshHeading | pubmed-meshheading:17428094... | lld:pubmed |
| pubmed-article:17428094 | pubmed:meshHeading | pubmed-meshheading:17428094... | lld:pubmed |
| pubmed-article:17428094 | pubmed:meshHeading | pubmed-meshheading:17428094... | lld:pubmed |
| pubmed-article:17428094 | pubmed:meshHeading | pubmed-meshheading:17428094... | lld:pubmed |
| pubmed-article:17428094 | pubmed:meshHeading | pubmed-meshheading:17428094... | lld:pubmed |
| pubmed-article:17428094 | pubmed:meshHeading | pubmed-meshheading:17428094... | lld:pubmed |
| pubmed-article:17428094 | pubmed:meshHeading | pubmed-meshheading:17428094... | lld:pubmed |
| pubmed-article:17428094 | pubmed:meshHeading | pubmed-meshheading:17428094... | lld:pubmed |
| pubmed-article:17428094 | pubmed:year | 2007 | lld:pubmed |
| pubmed-article:17428094 | pubmed:articleTitle | Synthesis and binding studies of Alzheimer ligands on solid support. | lld:pubmed |
| pubmed-article:17428094 | pubmed:affiliation | Fachbereich Chemie, Universität Marburg, Hans-Meerwein-Strasse, 35032 Marburg, Germany. | lld:pubmed |
| pubmed-article:17428094 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:17428094 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
