| pubmed-article:17367872 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:17367872 | lifeskim:mentions | umls-concept:C0019704 | lld:lifeskim |
| pubmed-article:17367872 | lifeskim:mentions | umls-concept:C0032105 | lld:lifeskim |
| pubmed-article:17367872 | lifeskim:mentions | umls-concept:C0035668 | lld:lifeskim |
| pubmed-article:17367872 | lifeskim:mentions | umls-concept:C0599161 | lld:lifeskim |
| pubmed-article:17367872 | lifeskim:mentions | umls-concept:C0679932 | lld:lifeskim |
| pubmed-article:17367872 | lifeskim:mentions | umls-concept:C1510438 | lld:lifeskim |
| pubmed-article:17367872 | lifeskim:mentions | umls-concept:C0449560 | lld:lifeskim |
| pubmed-article:17367872 | lifeskim:mentions | umls-concept:C1709793 | lld:lifeskim |
| pubmed-article:17367872 | lifeskim:mentions | umls-concept:C0205352 | lld:lifeskim |
| pubmed-article:17367872 | pubmed:issue | 1-2 | lld:pubmed |
| pubmed-article:17367872 | pubmed:dateCreated | 2007-4-23 | lld:pubmed |
| pubmed-article:17367872 | pubmed:abstractText | An easy, inexpensive competitive RT-PCR assay for HIV-1 RNA quantitation was constructed. A 138-bp sequence in the HIV-1 gag p24 region was selected as the target and co-amplified with competitor RNA containing an internal 44-bp deletion. Quantitation of serial dilutions of control RNA samples prepared from the LAI isolate demonstrated a good linearity (R(2)=0.991) within the range between 10 and 250 copies/sample. The detection limit of the assay was determined to be 3.8 copies/sample by Probit analysis and corresponded to 110 copies/ml in plasma. The intra-assay CV value was 9.1%, and the inter-assay value was 25.9%. Both were comparable to those obtained with commercially available HIV-1 RNA quantitation kits. The correlation efficient for the results obtained in 47 plasma samples from HIV-1-infected individuals (subtype A in 1, subtype B in 25, subtype C in 4, subtype F in 1, and CRF01 AE in 16) with the competitive RT-PCR and Cobas Amplicor HIV-1 Monitor test v1.5 was 0.956 for subtype B and 0.947 for subtype non-B. The assay devised is a good alternative for monitoring antiretroviral therapy in resource-poor countries. | lld:pubmed |
| pubmed-article:17367872 | pubmed:language | eng | lld:pubmed |
| pubmed-article:17367872 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17367872 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:17367872 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17367872 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17367872 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17367872 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:17367872 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:17367872 | pubmed:issn | 0166-0934 | lld:pubmed |
| pubmed-article:17367872 | pubmed:author | pubmed-author:YamamotoNaoki... | lld:pubmed |
| pubmed-article:17367872 | pubmed:author | pubmed-author:SugiuraWataru... | lld:pubmed |
| pubmed-article:17367872 | pubmed:author | pubmed-author:KatoShingoS | lld:pubmed |
| pubmed-article:17367872 | pubmed:author | pubmed-author:NishizawaMasa... | lld:pubmed |
| pubmed-article:17367872 | pubmed:author | pubmed-author:HamatakeMakik... | lld:pubmed |
| pubmed-article:17367872 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:17367872 | pubmed:volume | 142 | lld:pubmed |
| pubmed-article:17367872 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:17367872 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:17367872 | pubmed:pagination | 113-7 | lld:pubmed |
| pubmed-article:17367872 | pubmed:meshHeading | pubmed-meshheading:17367872... | lld:pubmed |
| pubmed-article:17367872 | pubmed:meshHeading | pubmed-meshheading:17367872... | lld:pubmed |
| pubmed-article:17367872 | pubmed:meshHeading | pubmed-meshheading:17367872... | lld:pubmed |
| pubmed-article:17367872 | pubmed:meshHeading | pubmed-meshheading:17367872... | lld:pubmed |
| pubmed-article:17367872 | pubmed:meshHeading | pubmed-meshheading:17367872... | lld:pubmed |
| pubmed-article:17367872 | pubmed:meshHeading | pubmed-meshheading:17367872... | lld:pubmed |
| pubmed-article:17367872 | pubmed:meshHeading | pubmed-meshheading:17367872... | lld:pubmed |
| pubmed-article:17367872 | pubmed:meshHeading | pubmed-meshheading:17367872... | lld:pubmed |
| pubmed-article:17367872 | pubmed:meshHeading | pubmed-meshheading:17367872... | lld:pubmed |
| pubmed-article:17367872 | pubmed:meshHeading | pubmed-meshheading:17367872... | lld:pubmed |
| pubmed-article:17367872 | pubmed:year | 2007 | lld:pubmed |
| pubmed-article:17367872 | pubmed:articleTitle | A simple competitive RT-PCR assay for quantitation of HIV-1 subtype B and non-B RNA in plasma. | lld:pubmed |
| pubmed-article:17367872 | pubmed:affiliation | Department of Molecular Virology, Tokyo Medical Dental University, Bunkyo, Tokyo 1138510, Japan. | lld:pubmed |
| pubmed-article:17367872 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:17367872 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:17367872 | pubmed:publicationType | Evaluation Studies | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17367872 | lld:pubmed |
