| pubmed-article:17349828 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:17349828 | lifeskim:mentions | umls-concept:C0005889 | lld:lifeskim |
| pubmed-article:17349828 | lifeskim:mentions | umls-concept:C1521806 | lld:lifeskim |
| pubmed-article:17349828 | lifeskim:mentions | umls-concept:C0680730 | lld:lifeskim |
| pubmed-article:17349828 | lifeskim:mentions | umls-concept:C1384115 | lld:lifeskim |
| pubmed-article:17349828 | lifeskim:mentions | umls-concept:C0444706 | lld:lifeskim |
| pubmed-article:17349828 | lifeskim:mentions | umls-concept:C0699857 | lld:lifeskim |
| pubmed-article:17349828 | lifeskim:mentions | umls-concept:C1148554 | lld:lifeskim |
| pubmed-article:17349828 | lifeskim:mentions | umls-concept:C0392762 | lld:lifeskim |
| pubmed-article:17349828 | lifeskim:mentions | umls-concept:C0205460 | lld:lifeskim |
| pubmed-article:17349828 | lifeskim:mentions | umls-concept:C1958799 | lld:lifeskim |
| pubmed-article:17349828 | lifeskim:mentions | umls-concept:C0243072 | lld:lifeskim |
| pubmed-article:17349828 | pubmed:issue | 1-2 | lld:pubmed |
| pubmed-article:17349828 | pubmed:dateCreated | 2007-5-15 | lld:pubmed |
| pubmed-article:17349828 | pubmed:abstractText | The nitrated lipids 9-nitro-oleic acid (9-NO(2)-OA) and 10-nitro-oleic acid (10-NO(2)-OA) have been reported to be present in blood of healthy humans. Free and esterified forms of 9-NO(2)-OA and 10-NO(2)-OA have been detected in human plasma at about 600 and 300 nM, respectively. These concentrations are of the same order of magnitude of circulating nitrite. In theory, 9-NO(2)-OA and 10-NO(2)-OA may interfere with the analysis of circulating nitrite and nitrate. In the present study, we investigated a possible interference of 9-NO(2)-OA and 10-NO(2)-OA with the GC-MS method of analysis of nitrite and nitrate involving derivatization by pentafluorobenzyl (PFB) bromide in aqueous acetone at 50 degrees C for 5 min (nitrite) or for 60 min (nitrite and nitrate). Our results show that 9-NO(2)-OA and 10-NO(2)-OA do not interfere with the GC-MS analysis of nitrite and nitrate as PFB derivatives in plasma and phosphate buffered saline when added to these matrices at supraphysiological concentrations of 1-10 microM. Thus, nitrated lipids such as 9-NO(2)-OA and 10-NO(2)-OA can be excluded as potential interfering substances in the GC-MS quantitative determination of nitrite and nitrate as their PFB derivatives. | lld:pubmed |
| pubmed-article:17349828 | pubmed:language | eng | lld:pubmed |
| pubmed-article:17349828 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17349828 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:17349828 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17349828 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17349828 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17349828 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17349828 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17349828 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17349828 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17349828 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:17349828 | pubmed:month | May | lld:pubmed |
| pubmed-article:17349828 | pubmed:issn | 1570-0232 | lld:pubmed |
| pubmed-article:17349828 | pubmed:author | pubmed-author:TsikasDimitri... | lld:pubmed |
| pubmed-article:17349828 | pubmed:author | pubmed-author:GutzkiFrank-M... | lld:pubmed |
| pubmed-article:17349828 | pubmed:author | pubmed-author:MitschkeAnjaA | lld:pubmed |
| pubmed-article:17349828 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:17349828 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:17349828 | pubmed:volume | 851 | lld:pubmed |
| pubmed-article:17349828 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:17349828 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:17349828 | pubmed:pagination | 287-91 | lld:pubmed |
| pubmed-article:17349828 | pubmed:meshHeading | pubmed-meshheading:17349828... | lld:pubmed |
| pubmed-article:17349828 | pubmed:meshHeading | pubmed-meshheading:17349828... | lld:pubmed |
| pubmed-article:17349828 | pubmed:meshHeading | pubmed-meshheading:17349828... | lld:pubmed |
| pubmed-article:17349828 | pubmed:meshHeading | pubmed-meshheading:17349828... | lld:pubmed |
| pubmed-article:17349828 | pubmed:meshHeading | pubmed-meshheading:17349828... | lld:pubmed |
| pubmed-article:17349828 | pubmed:meshHeading | pubmed-meshheading:17349828... | lld:pubmed |
| pubmed-article:17349828 | pubmed:meshHeading | pubmed-meshheading:17349828... | lld:pubmed |
| pubmed-article:17349828 | pubmed:meshHeading | pubmed-meshheading:17349828... | lld:pubmed |
| pubmed-article:17349828 | pubmed:meshHeading | pubmed-meshheading:17349828... | lld:pubmed |
| pubmed-article:17349828 | pubmed:year | 2007 | lld:pubmed |
| pubmed-article:17349828 | pubmed:articleTitle | 9- and 10-Nitro-oleic acid do not interfere with the GC-MS quantitative determination of nitrite and nitrate in biological fluids when measured as their pentafluorobenzyl derivatives. | lld:pubmed |
| pubmed-article:17349828 | pubmed:affiliation | Institut für Klinische Pharmakologie, Medizinische Hochschule Hannover, Carl-Neuberg-Strasse 1, D-30625 Hannover, Germany. | lld:pubmed |
| pubmed-article:17349828 | pubmed:publicationType | Journal Article | lld:pubmed |
