| pubmed-article:1734919 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1734919 | lifeskim:mentions | umls-concept:C0025663 | lld:lifeskim |
| pubmed-article:1734919 | lifeskim:mentions | umls-concept:C0023690 | lld:lifeskim |
| pubmed-article:1734919 | lifeskim:mentions | umls-concept:C0032520 | lld:lifeskim |
| pubmed-article:1734919 | lifeskim:mentions | umls-concept:C1514468 | lld:lifeskim |
| pubmed-article:1734919 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:1734919 | pubmed:dateCreated | 1992-3-12 | lld:pubmed |
| pubmed-article:1734919 | pubmed:abstractText | This report presents data demonstrating a simple method that can potentially be extended to a wide range of cloning strategies to increase the yield of insert-containing recombinants. The method requires that the ligation of an insert to a vector does not regenerate the original restriction enzyme recognition sequence. In the example presented, PCR products were blunt-end ligated to a SmaI-cut vector, in the presence of SmaI endonuclease. The addition of the restriction enzyme to the ligation reaction dramatically favored the ligation of insert to vector rather than vector self-ligation. | lld:pubmed |
| pubmed-article:1734919 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1734919 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1734919 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1734919 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1734919 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1734919 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1734919 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1734919 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:1734919 | pubmed:issn | 0736-6205 | lld:pubmed |
| pubmed-article:1734919 | pubmed:author | pubmed-author:SchwartzL MLM | lld:pubmed |
| pubmed-article:1734919 | pubmed:author | pubmed-author:LiuZ GZG | lld:pubmed |
| pubmed-article:1734919 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1734919 | pubmed:volume | 12 | lld:pubmed |
| pubmed-article:1734919 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1734919 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1734919 | pubmed:pagination | 28, 30 | lld:pubmed |
| pubmed-article:1734919 | pubmed:dateRevised | 2008-8-29 | lld:pubmed |
| pubmed-article:1734919 | pubmed:meshHeading | pubmed-meshheading:1734919-... | lld:pubmed |
| pubmed-article:1734919 | pubmed:meshHeading | pubmed-meshheading:1734919-... | lld:pubmed |
| pubmed-article:1734919 | pubmed:meshHeading | pubmed-meshheading:1734919-... | lld:pubmed |
| pubmed-article:1734919 | pubmed:meshHeading | pubmed-meshheading:1734919-... | lld:pubmed |
| pubmed-article:1734919 | pubmed:meshHeading | pubmed-meshheading:1734919-... | lld:pubmed |
| pubmed-article:1734919 | pubmed:meshHeading | pubmed-meshheading:1734919-... | lld:pubmed |
| pubmed-article:1734919 | pubmed:meshHeading | pubmed-meshheading:1734919-... | lld:pubmed |
| pubmed-article:1734919 | pubmed:meshHeading | pubmed-meshheading:1734919-... | lld:pubmed |
| pubmed-article:1734919 | pubmed:year | 1992 | lld:pubmed |
| pubmed-article:1734919 | pubmed:articleTitle | An efficient method for blunt-end ligation of PCR products. | lld:pubmed |
| pubmed-article:1734919 | pubmed:affiliation | Molecular and Cellular Biology Program, Morrill Science Center, University of Massachusetts, Amherst 01003. | lld:pubmed |
| pubmed-article:1734919 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1734919 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1734919 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1734919 | lld:pubmed |
