| pubmed-article:17344056 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:17344056 | lifeskim:mentions | umls-concept:C0032098 | lld:lifeskim |
| pubmed-article:17344056 | lifeskim:mentions | umls-concept:C0024643 | lld:lifeskim |
| pubmed-article:17344056 | lifeskim:mentions | umls-concept:C0220825 | lld:lifeskim |
| pubmed-article:17344056 | lifeskim:mentions | umls-concept:C0023775 | lld:lifeskim |
| pubmed-article:17344056 | lifeskim:mentions | umls-concept:C1514468 | lld:lifeskim |
| pubmed-article:17344056 | lifeskim:mentions | umls-concept:C1510438 | lld:lifeskim |
| pubmed-article:17344056 | lifeskim:mentions | umls-concept:C0048297 | lld:lifeskim |
| pubmed-article:17344056 | lifeskim:mentions | umls-concept:C1957596 | lld:lifeskim |
| pubmed-article:17344056 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:17344056 | pubmed:dateCreated | 2007-3-26 | lld:pubmed |
| pubmed-article:17344056 | pubmed:abstractText | The 1-methyl-2-phenylindole colorimetric assay is considered specific for malondialdehyde (MDA) and 4-hydroxynonenal (HNE) in mammalian systems, but its specificity in plant tissues is unknown. This study demonstrates that the assay produces a purple/blue chromophore with an absorbance peak at 586 nm for a malondialdehyde standard, while aqueous extractions from Ribes spp. Beta vulgaris, and Lycopersicon esculentum tissues produce an orange chromophore with an absorbance maximum at 450 nm and a large shoulder that extends to 700 nm. No distinctive MDA peak was discernable in plant samples at lambda=586 nm and absorbance was attributed to background interference. The reaction between sucrose and 1-methyl-2-phenylindole produced an orange chromophore with a spectrum similar to those obtained from plant extractions, suggesting that simple sugars are the likely source of background interference. This study demonstrates that the 1-methyl-2-phenylindole colorimetric assay is non-specific for detecting MDA and HNE in plants and its use is cautioned due to interference, particularly from sugars. | lld:pubmed |
| pubmed-article:17344056 | pubmed:language | eng | lld:pubmed |
| pubmed-article:17344056 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17344056 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:17344056 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17344056 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17344056 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17344056 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17344056 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17344056 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17344056 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17344056 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17344056 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17344056 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:17344056 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:17344056 | pubmed:issn | 0981-9428 | lld:pubmed |
| pubmed-article:17344056 | pubmed:author | pubmed-author:HardingKeithK | lld:pubmed |
| pubmed-article:17344056 | pubmed:author | pubmed-author:BensonErica... | lld:pubmed |
| pubmed-article:17344056 | pubmed:author | pubmed-author:JohnstonJason... | lld:pubmed |
| pubmed-article:17344056 | pubmed:author | pubmed-author:HorneSusanS | lld:pubmed |
| pubmed-article:17344056 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:17344056 | pubmed:volume | 45 | lld:pubmed |
| pubmed-article:17344056 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:17344056 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:17344056 | pubmed:pagination | 108-12 | lld:pubmed |
| pubmed-article:17344056 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
| pubmed-article:17344056 | pubmed:meshHeading | pubmed-meshheading:17344056... | lld:pubmed |
| pubmed-article:17344056 | pubmed:meshHeading | pubmed-meshheading:17344056... | lld:pubmed |
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| pubmed-article:17344056 | pubmed:meshHeading | pubmed-meshheading:17344056... | lld:pubmed |
| pubmed-article:17344056 | pubmed:meshHeading | pubmed-meshheading:17344056... | lld:pubmed |
| pubmed-article:17344056 | pubmed:meshHeading | pubmed-meshheading:17344056... | lld:pubmed |
| pubmed-article:17344056 | pubmed:meshHeading | pubmed-meshheading:17344056... | lld:pubmed |
| pubmed-article:17344056 | pubmed:meshHeading | pubmed-meshheading:17344056... | lld:pubmed |
| pubmed-article:17344056 | pubmed:meshHeading | pubmed-meshheading:17344056... | lld:pubmed |
| pubmed-article:17344056 | pubmed:meshHeading | pubmed-meshheading:17344056... | lld:pubmed |
| pubmed-article:17344056 | pubmed:meshHeading | pubmed-meshheading:17344056... | lld:pubmed |
| pubmed-article:17344056 | pubmed:meshHeading | pubmed-meshheading:17344056... | lld:pubmed |
| pubmed-article:17344056 | pubmed:meshHeading | pubmed-meshheading:17344056... | lld:pubmed |
| pubmed-article:17344056 | pubmed:year | 2007 | lld:pubmed |
| pubmed-article:17344056 | pubmed:articleTitle | Evaluation of the 1-methyl-2-phenylindole colorimetric assay for aldehydic lipid peroxidation products in plants: malondialdehyde and 4-hydroxynonenal. | lld:pubmed |
| pubmed-article:17344056 | pubmed:affiliation | Plant Conservation Group, School of Contemporary Science, University of Abertay Dundee, Kydd Building, Bell St, Dundee DD1 1HG, UK. jjohnston@hortresarch.co.nz | lld:pubmed |
| pubmed-article:17344056 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:17344056 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
