| pubmed-article:1733774 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1733774 | lifeskim:mentions | umls-concept:C0014834 | lld:lifeskim |
| pubmed-article:1733774 | lifeskim:mentions | umls-concept:C1882726 | lld:lifeskim |
| pubmed-article:1733774 | lifeskim:mentions | umls-concept:C0023779 | lld:lifeskim |
| pubmed-article:1733774 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:1733774 | lifeskim:mentions | umls-concept:C1171362 | lld:lifeskim |
| pubmed-article:1733774 | lifeskim:mentions | umls-concept:C0441655 | lld:lifeskim |
| pubmed-article:1733774 | lifeskim:mentions | umls-concept:C0205370 | lld:lifeskim |
| pubmed-article:1733774 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:1733774 | lifeskim:mentions | umls-concept:C1515670 | lld:lifeskim |
| pubmed-article:1733774 | lifeskim:mentions | umls-concept:C1705822 | lld:lifeskim |
| pubmed-article:1733774 | lifeskim:mentions | umls-concept:C0376315 | lld:lifeskim |
| pubmed-article:1733774 | lifeskim:mentions | umls-concept:C0348011 | lld:lifeskim |
| pubmed-article:1733774 | lifeskim:mentions | umls-concept:C1709634 | lld:lifeskim |
| pubmed-article:1733774 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:1733774 | pubmed:dateCreated | 1992-3-4 | lld:pubmed |
| pubmed-article:1733774 | pubmed:abstractText | The cDNA encoding the precursor form of non-specific lipid-transfer protein (pre-nsL-TP) from rat liver was cloned into the expression vector pET3d. The resulting plasmid was transformed to the Escherichia coli strain BL21(DE3). After induction of the bacteria with isopropyl-beta-D-thiogalactopyranoside (IPTG) pre-nsL-TP was purified from the bacterial lysate by anion exchange chromatography followed by gelfiltration. From 11 of culture, 6-7 mg of pre-nsL-TP was obtained, equal to approximately 7% of the cytoplasmic protein. By use of a fluorescence lipid transfer assay, pre-nsL-TP was found to have lipid transfer activity identical to mature nsL-TP. | lld:pubmed |
| pubmed-article:1733774 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1733774 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1733774 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1733774 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1733774 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1733774 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1733774 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1733774 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1733774 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1733774 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1733774 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1733774 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:1733774 | pubmed:issn | 0014-5793 | lld:pubmed |
| pubmed-article:1733774 | pubmed:author | pubmed-author:WirtzK WKW | lld:pubmed |
| pubmed-article:1733774 | pubmed:author | pubmed-author:OssendorpB... | lld:pubmed |
| pubmed-article:1733774 | pubmed:author | pubmed-author:GeijtenbeekT... | lld:pubmed |
| pubmed-article:1733774 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1733774 | pubmed:day | 20 | lld:pubmed |
| pubmed-article:1733774 | pubmed:volume | 296 | lld:pubmed |
| pubmed-article:1733774 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1733774 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1733774 | pubmed:pagination | 179-83 | lld:pubmed |
| pubmed-article:1733774 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:1733774 | pubmed:meshHeading | pubmed-meshheading:1733774-... | lld:pubmed |
| pubmed-article:1733774 | pubmed:meshHeading | pubmed-meshheading:1733774-... | lld:pubmed |
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| pubmed-article:1733774 | pubmed:meshHeading | pubmed-meshheading:1733774-... | lld:pubmed |
| pubmed-article:1733774 | pubmed:meshHeading | pubmed-meshheading:1733774-... | lld:pubmed |
| pubmed-article:1733774 | pubmed:meshHeading | pubmed-meshheading:1733774-... | lld:pubmed |
| pubmed-article:1733774 | pubmed:meshHeading | pubmed-meshheading:1733774-... | lld:pubmed |
| pubmed-article:1733774 | pubmed:meshHeading | pubmed-meshheading:1733774-... | lld:pubmed |
| pubmed-article:1733774 | pubmed:meshHeading | pubmed-meshheading:1733774-... | lld:pubmed |
| pubmed-article:1733774 | pubmed:meshHeading | pubmed-meshheading:1733774-... | lld:pubmed |
| pubmed-article:1733774 | pubmed:meshHeading | pubmed-meshheading:1733774-... | lld:pubmed |
| pubmed-article:1733774 | pubmed:meshHeading | pubmed-meshheading:1733774-... | lld:pubmed |
| pubmed-article:1733774 | pubmed:year | 1992 | lld:pubmed |
| pubmed-article:1733774 | pubmed:articleTitle | The precursor form of the rat liver non-specific lipid-transfer protein, expressed in Escherichia coli, has lipid transfer activity. | lld:pubmed |
| pubmed-article:1733774 | pubmed:affiliation | Centre for Biomembranes and Lipid Enzymology, State University of Utrecht, The Netherlands. | lld:pubmed |
| pubmed-article:1733774 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1733774 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1733774 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1733774 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1733774 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1733774 | lld:pubmed |
