| pubmed-article:17332927 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:17332927 | lifeskim:mentions | umls-concept:C0017296 | lld:lifeskim |
| pubmed-article:17332927 | lifeskim:mentions | umls-concept:C0162638 | lld:lifeskim |
| pubmed-article:17332927 | lifeskim:mentions | umls-concept:C0302600 | lld:lifeskim |
| pubmed-article:17332927 | lifeskim:mentions | umls-concept:C0219474 | lld:lifeskim |
| pubmed-article:17332927 | lifeskim:mentions | umls-concept:C0376515 | lld:lifeskim |
| pubmed-article:17332927 | lifeskim:mentions | umls-concept:C1527249 | lld:lifeskim |
| pubmed-article:17332927 | lifeskim:mentions | umls-concept:C0442805 | lld:lifeskim |
| pubmed-article:17332927 | lifeskim:mentions | umls-concept:C1149301 | lld:lifeskim |
| pubmed-article:17332927 | lifeskim:mentions | umls-concept:C0547047 | lld:lifeskim |
| pubmed-article:17332927 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:17332927 | pubmed:dateCreated | 2007-3-2 | lld:pubmed |
| pubmed-article:17332927 | pubmed:abstractText | Insulin-like growth factors are known to inhibit apoptosis and promote tumour angiogenesis. Previously we have shown that insulin-like growth factor binding protein-4 (IGFBP-4) gene therapy increased apoptosis and decreased mitosis in colon cancer. In this experiment we used HT-29 colon cancer cells to induce subcutaneous cancers in nude mice and administered either the mammalian expression vector with IGFBP-4 insert or vector only around the tumour site. Three weeks after gene transfer, tumours were harvested and expressions of Bax, Bcl-2 and IGF-I receptor in tumours were determined by Western blotting and immunofluorescence. Micro-vessel counting was performed by immunostaining with CD34 and von Willebrand antibodies. Results showed that tumours treated with IGFBP-4 gene had higher expression of Bax, lower expression of Bcl-2 and IGF-I receptor. Bcl-2 was localised to tumour cell cytoplasm while Bax was expressed both in the interstitial area and cytoplasm. IGFBP-4 treatment also decreased micro-vessel count in tumour tissues. Micro-vessels were mainly located in the periphery and interstitial area. This experiment shows that IGFBP-4 gene therapy increases tumour apoptosis via altering the expressions of Bcl-2 and Bax and decreasing the angiogenesis in colorectal cancer. | lld:pubmed |
| pubmed-article:17332927 | pubmed:language | eng | lld:pubmed |
| pubmed-article:17332927 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17332927 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:17332927 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17332927 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17332927 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17332927 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:17332927 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:17332927 | pubmed:issn | 1019-6439 | lld:pubmed |
| pubmed-article:17332927 | pubmed:author | pubmed-author:SeifalianAlex... | lld:pubmed |
| pubmed-article:17332927 | pubmed:author | pubmed-author:GoldspinkGeof... | lld:pubmed |
| pubmed-article:17332927 | pubmed:author | pubmed-author:YangShi YuSY | lld:pubmed |
| pubmed-article:17332927 | pubmed:author | pubmed-author:WinsletMarc... | lld:pubmed |
| pubmed-article:17332927 | pubmed:author | pubmed-author:SalesKevin... | lld:pubmed |
| pubmed-article:17332927 | pubmed:author | pubmed-author:DuraiRajarama... | lld:pubmed |
| pubmed-article:17332927 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:17332927 | pubmed:volume | 30 | lld:pubmed |
| pubmed-article:17332927 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:17332927 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:17332927 | pubmed:pagination | 883-8 | lld:pubmed |
| pubmed-article:17332927 | pubmed:meshHeading | pubmed-meshheading:17332927... | lld:pubmed |
| pubmed-article:17332927 | pubmed:meshHeading | pubmed-meshheading:17332927... | lld:pubmed |
| pubmed-article:17332927 | pubmed:meshHeading | pubmed-meshheading:17332927... | lld:pubmed |
| pubmed-article:17332927 | pubmed:meshHeading | pubmed-meshheading:17332927... | lld:pubmed |
| pubmed-article:17332927 | pubmed:meshHeading | pubmed-meshheading:17332927... | lld:pubmed |
| pubmed-article:17332927 | pubmed:meshHeading | pubmed-meshheading:17332927... | lld:pubmed |
| pubmed-article:17332927 | pubmed:meshHeading | pubmed-meshheading:17332927... | lld:pubmed |
| pubmed-article:17332927 | pubmed:meshHeading | pubmed-meshheading:17332927... | lld:pubmed |
| pubmed-article:17332927 | pubmed:meshHeading | pubmed-meshheading:17332927... | lld:pubmed |
| pubmed-article:17332927 | pubmed:meshHeading | pubmed-meshheading:17332927... | lld:pubmed |
| pubmed-article:17332927 | pubmed:year | 2007 | lld:pubmed |
| pubmed-article:17332927 | pubmed:articleTitle | Insulin-like growth factor binding protein-4 gene therapy increases apoptosis by altering Bcl-2 and Bax proteins and decreases angiogenesis in colorectal cancer. | lld:pubmed |
| pubmed-article:17332927 | pubmed:affiliation | Academic Division of Surgical and Interventional Sciences, University College London, Hampstead Campus, and Royal Free Hampstead NHS Trust Hospital, London NW3 2FP, UK. | lld:pubmed |
| pubmed-article:17332927 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17332927 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17332927 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17332927 | lld:pubmed |
