| pubmed-article:17313661 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:17313661 | lifeskim:mentions | umls-concept:C0004611 | lld:lifeskim |
| pubmed-article:17313661 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:17313661 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:17313661 | lifeskim:mentions | umls-concept:C0021853 | lld:lifeskim |
| pubmed-article:17313661 | lifeskim:mentions | umls-concept:C1515654 | lld:lifeskim |
| pubmed-article:17313661 | lifeskim:mentions | umls-concept:C0022262 | lld:lifeskim |
| pubmed-article:17313661 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:17313661 | pubmed:dateCreated | 2007-3-26 | lld:pubmed |
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| pubmed-article:17313661 | pubmed:abstractText | 16S rRNA-based stable isotope probing (SIP) and nuclear magnetic resonance (NMR) spectroscopy-based metabolic profiling were used to identify bacteria fermenting glucose under conditions simulating the human intestine. The TIM-2 in vitro model of the human intestine was inoculated with a GI tract microbiota resembling that of the small intestine, to which subsequently 4, 20 or 40 mM of [U-(13)C]-glucose were added. RNA was extracted from lumen samples after 0 (control), 1, 2 and 4 h and subjected to density-gradient ultracentrifugation. Phylogenetic analysis of unlabeled 16S rRNA revealed a microbial community dominated by lactic acid bacteria and Clostridium perfringens. Distinct (13)C-incorporation into bacterial RNA was only observed for the 40-mM addition. 16S rRNA fingerprinting showed an activity drop of Lactobacillus fermentum after glucose addition, while Streptococcus bovis and C. perfringens were identified as the most active glucose-fermenters. Accordingly, NMR analysis identified lactate, acetate, butyrate and formate as the principal fermentation products, constituting up to 91% of the (13)C-carbon balance. RNA-SIP combined with metabolic profiling allowed us to detect differential utilization of a general model carbohydrate, indicating that this approach holds great potential to identify bacteria involved in the fermentation of dietary relevant oligo- and polymeric carbohydrates in the human intestine. | lld:pubmed |
| pubmed-article:17313661 | pubmed:language | eng | lld:pubmed |
| pubmed-article:17313661 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17313661 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:17313661 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17313661 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:17313661 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:17313661 | pubmed:issn | 0168-6496 | lld:pubmed |
| pubmed-article:17313661 | pubmed:author | pubmed-author:de... | lld:pubmed |
| pubmed-article:17313661 | pubmed:author | pubmed-author:DeutzNicolaas... | lld:pubmed |
| pubmed-article:17313661 | pubmed:author | pubmed-author:PluggeCarolin... | lld:pubmed |
| pubmed-article:17313661 | pubmed:author | pubmed-author:DijkemaCorC | lld:pubmed |
| pubmed-article:17313661 | pubmed:author | pubmed-author:de... | lld:pubmed |
| pubmed-article:17313661 | pubmed:author | pubmed-author:de... | lld:pubmed |
| pubmed-article:17313661 | pubmed:author | pubmed-author:EgertMarkusM | lld:pubmed |
| pubmed-article:17313661 | pubmed:author | pubmed-author:SmidtHaukeH | lld:pubmed |
| pubmed-article:17313661 | pubmed:author | pubmed-author:VenemaKoenK | lld:pubmed |
| pubmed-article:17313661 | pubmed:author | pubmed-author:MaathuisAnnet... | lld:pubmed |
| pubmed-article:17313661 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:17313661 | pubmed:volume | 60 | lld:pubmed |
| pubmed-article:17313661 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:17313661 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:17313661 | pubmed:pagination | 126-35 | lld:pubmed |
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| pubmed-article:17313661 | pubmed:meshHeading | pubmed-meshheading:17313661... | lld:pubmed |
| pubmed-article:17313661 | pubmed:meshHeading | pubmed-meshheading:17313661... | lld:pubmed |
| pubmed-article:17313661 | pubmed:meshHeading | pubmed-meshheading:17313661... | lld:pubmed |
| pubmed-article:17313661 | pubmed:meshHeading | pubmed-meshheading:17313661... | lld:pubmed |
| pubmed-article:17313661 | pubmed:meshHeading | pubmed-meshheading:17313661... | lld:pubmed |
| pubmed-article:17313661 | pubmed:year | 2007 | lld:pubmed |
| pubmed-article:17313661 | pubmed:articleTitle | Identification of glucose-fermenting bacteria present in an in vitro model of the human intestine by RNA-stable isotope probing. | lld:pubmed |
| pubmed-article:17313661 | pubmed:affiliation | Wageningen Centre for Food Sciences, Wageningen, The Netherlands. | lld:pubmed |
| pubmed-article:17313661 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:17313661 | pubmed:publicationType | Evaluation Studies | lld:pubmed |
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