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pubmed-article:17306763pubmed:abstractTextPhysiological and behavioral circadian rhythms in mammals are orchestrated by a central circadian clock located in the suprachiasmatic nucleus (SCN) of the hypothalamus. Photic input entrains the phase of the central clock, and many peripheral clocks are regulated by neural or hormonal output from the SCN. We established cell lines derived from the rat embryonic SCN to examine the molecular network of the central clock. An established cell line exhibited the stable circadian expression of clock genes. The circadian oscillation was abruptly phase-shifted by forskolin, and abolished by siBmal1. These results are compatible with in vivo studies of the SCN.lld:pubmed
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pubmed-article:17306763pubmed:year2007lld:pubmed
pubmed-article:17306763pubmed:articleTitleEstablishment of cell lines derived from the rat suprachiasmatic nucleus.lld:pubmed
pubmed-article:17306763pubmed:affiliationResearch Group of Chronogenomics, Mitsubishi Kagaku Institute of Life Sciences, 11 Minamiooya, Machida, Tokyo 194-8511, Japan.lld:pubmed
pubmed-article:17306763pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:17306763pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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