| pubmed-article:17298035 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:17298035 | lifeskim:mentions | umls-concept:C0013018 | lld:lifeskim |
| pubmed-article:17298035 | lifeskim:mentions | umls-concept:C1335268 | lld:lifeskim |
| pubmed-article:17298035 | lifeskim:mentions | umls-concept:C0012854 | lld:lifeskim |
| pubmed-article:17298035 | lifeskim:mentions | umls-concept:C0441655 | lld:lifeskim |
| pubmed-article:17298035 | lifeskim:mentions | umls-concept:C1948023 | lld:lifeskim |
| pubmed-article:17298035 | lifeskim:mentions | umls-concept:C0205269 | lld:lifeskim |
| pubmed-article:17298035 | lifeskim:mentions | umls-concept:C0917728 | lld:lifeskim |
| pubmed-article:17298035 | pubmed:issue | 10 | lld:pubmed |
| pubmed-article:17298035 | pubmed:dateCreated | 2007-3-6 | lld:pubmed |
| pubmed-article:17298035 | pubmed:abstractText | LEDGF/p75 is known to enhance the integrase strand transfer activity in vitro, but the underlying mechanism is unclear. Using an integrase assay with a chemiluminescent readout adapted to a 96-well plate format, the effect of LEDGF/p75 on both the 3'-processing and strand transfer steps was analyzed. Integrase inhibitors of the strand transfer reaction remained active in the presence of LEDGF/p75, but displayed 3- to 7-fold higher IC50 values. Our analyses indicate that, in the presence of 150 nM LEDGF/p75, active integrase/donor DNA complexes were increased by 5.3-fold during the 3'-processing step. In addition, these integrase/donor DNA complexes showed a 4.5-fold greater affinity for the target DNA during the subsequent strand transfer step. We also observed a 3.7-fold increase in the rate constant of catalysis of the strand transfer step when 150 nM LEDGF/p75 was present during the 3'-processing step. In contrast, when LEDGF/p75 was added at the beginning of the strand transfer step, no increase in either the concentration of active integrase/donor DNA complex or its rate constant of strand transfer catalysis was observed. This observation suggested that the integrase/donor DNA formed in the absence of LEDGF/p75 became refractory to the stimulatory effect of LEDGF/p75. Instead, this LEDGF/p75 added at the start of the strand transfer step was able to promote the formation of a new cohort of active integrase/donor DNA complexes which became functional with a delay of 45 min after LEDGF/p75 addition. We propose a model whereby LEDGF/p75 can only bind integrase before the latter binds donor DNA whereas donor DNA can engage either free or LEDGF/p75-bound integrase. | lld:pubmed |
| pubmed-article:17298035 | pubmed:language | eng | lld:pubmed |
| pubmed-article:17298035 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17298035 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:17298035 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17298035 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17298035 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17298035 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17298035 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17298035 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:17298035 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:17298035 | pubmed:issn | 0006-2960 | lld:pubmed |
| pubmed-article:17298035 | pubmed:author | pubmed-author:FangYuY | lld:pubmed |
| pubmed-article:17298035 | pubmed:author | pubmed-author:LeavittStepha... | lld:pubmed |
| pubmed-article:17298035 | pubmed:author | pubmed-author:LiuXiaohongX | lld:pubmed |
| pubmed-article:17298035 | pubmed:author | pubmed-author:McDermottMart... | lld:pubmed |
| pubmed-article:17298035 | pubmed:author | pubmed-author:HungMagdelein... | lld:pubmed |
| pubmed-article:17298035 | pubmed:author | pubmed-author:TsiangManuelM | lld:pubmed |
| pubmed-article:17298035 | pubmed:author | pubmed-author:JonesGregg... | lld:pubmed |
| pubmed-article:17298035 | pubmed:author | pubmed-author:WagnerAlice... | lld:pubmed |
| pubmed-article:17298035 | pubmed:author | pubmed-author:MacArthurHoll... | lld:pubmed |
| pubmed-article:17298035 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:17298035 | pubmed:day | 13 | lld:pubmed |
| pubmed-article:17298035 | pubmed:volume | 46 | lld:pubmed |
| pubmed-article:17298035 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:17298035 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:17298035 | pubmed:pagination | 2899-908 | lld:pubmed |
| pubmed-article:17298035 | pubmed:meshHeading | pubmed-meshheading:17298035... | lld:pubmed |
| pubmed-article:17298035 | pubmed:meshHeading | pubmed-meshheading:17298035... | lld:pubmed |
| pubmed-article:17298035 | pubmed:meshHeading | pubmed-meshheading:17298035... | lld:pubmed |
| pubmed-article:17298035 | pubmed:meshHeading | pubmed-meshheading:17298035... | lld:pubmed |
| pubmed-article:17298035 | pubmed:meshHeading | pubmed-meshheading:17298035... | lld:pubmed |
| pubmed-article:17298035 | pubmed:meshHeading | pubmed-meshheading:17298035... | lld:pubmed |
| pubmed-article:17298035 | pubmed:year | 2007 | lld:pubmed |
| pubmed-article:17298035 | pubmed:articleTitle | HIV-1 integrase preassembled on donor DNA is refractory to activity stimulation by LEDGF/p75. | lld:pubmed |
| pubmed-article:17298035 | pubmed:affiliation | Gilead Sciences, 333 Lakeside Drive, Foster City, California 94404, USA. | lld:pubmed |
| pubmed-article:17298035 | pubmed:publicationType | Journal Article | lld:pubmed |
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