| pubmed-article:1725262 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1725262 | lifeskim:mentions | umls-concept:C0014442 | lld:lifeskim |
| pubmed-article:1725262 | lifeskim:mentions | umls-concept:C0035711 | lld:lifeskim |
| pubmed-article:1725262 | lifeskim:mentions | umls-concept:C0035719 | lld:lifeskim |
| pubmed-article:1725262 | lifeskim:mentions | umls-concept:C1704675 | lld:lifeskim |
| pubmed-article:1725262 | lifeskim:mentions | umls-concept:C0596988 | lld:lifeskim |
| pubmed-article:1725262 | lifeskim:mentions | umls-concept:C1537998 | lld:lifeskim |
| pubmed-article:1725262 | lifeskim:mentions | umls-concept:C0061493 | lld:lifeskim |
| pubmed-article:1725262 | lifeskim:mentions | umls-concept:C0182400 | lld:lifeskim |
| pubmed-article:1725262 | pubmed:issue | 12 | lld:pubmed |
| pubmed-article:1725262 | pubmed:dateCreated | 1992-5-13 | lld:pubmed |
| pubmed-article:1725262 | pubmed:abstractText | This paper focuses on several aspects of the specificity of mutants of Escherichia coli glutaminyl-tRNA synthetase (GlnRS) and tRNA(Gln). Temperature-sensitive mutants located in glnS, the gene for GlnRS, have been described previously. The mutations responsible for the temperature-sensitive phenotype were analyzed, and pseudorevertants of these mutants isolated and characterized. The nature of these mutations is discussed in terms of their location in the three-dimensional structure of the tRNA(Gln).GlnRS complex. In order to characterize the specificity of the aminoacylation reaction, mutant tRNA(Gln) species were synthesized with either a 2'-deoxy AMP or 3'-deoxy AMP as their 3'-terminal nucleotide. Subsequent assays for aminoacylation and ATP/PPi exchange activity established the esterification of glutamine to the 2'-hydroxyl of the terminal adenosine; there is no glutaminylation of the 3'-OH group. This correlates with the classification of GlnRS as a class I aminoacyl-tRNA synthetase. Mutations in tRNA(Gln) are discussed which affect the recognition of GlnRS and the current concept of glutamine identity in E coli is reviewed. | lld:pubmed |
| pubmed-article:1725262 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1725262 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1725262 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1725262 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1725262 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1725262 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1725262 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1725262 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:1725262 | pubmed:issn | 0300-9084 | lld:pubmed |
| pubmed-article:1725262 | pubmed:author | pubmed-author:SöllDD | lld:pubmed |
| pubmed-article:1725262 | pubmed:author | pubmed-author:RogersM JMJ | lld:pubmed |
| pubmed-article:1725262 | pubmed:author | pubmed-author:ConleyJJ | lld:pubmed |
| pubmed-article:1725262 | pubmed:author | pubmed-author:LeptakCC | lld:pubmed |
| pubmed-article:1725262 | pubmed:author | pubmed-author:PlumbridgeJJ | lld:pubmed |
| pubmed-article:1725262 | pubmed:author | pubmed-author:Englisch-Pete... | lld:pubmed |
| pubmed-article:1725262 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1725262 | pubmed:volume | 73 | lld:pubmed |
| pubmed-article:1725262 | pubmed:geneSymbol | glnS | lld:pubmed |
| pubmed-article:1725262 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1725262 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1725262 | pubmed:pagination | 1501-8 | lld:pubmed |
| pubmed-article:1725262 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:1725262 | pubmed:meshHeading | pubmed-meshheading:1725262-... | lld:pubmed |
| pubmed-article:1725262 | pubmed:meshHeading | pubmed-meshheading:1725262-... | lld:pubmed |
| pubmed-article:1725262 | pubmed:meshHeading | pubmed-meshheading:1725262-... | lld:pubmed |
| pubmed-article:1725262 | pubmed:meshHeading | pubmed-meshheading:1725262-... | lld:pubmed |
| pubmed-article:1725262 | pubmed:meshHeading | pubmed-meshheading:1725262-... | lld:pubmed |
| pubmed-article:1725262 | pubmed:meshHeading | pubmed-meshheading:1725262-... | lld:pubmed |
| pubmed-article:1725262 | pubmed:meshHeading | pubmed-meshheading:1725262-... | lld:pubmed |
| pubmed-article:1725262 | pubmed:meshHeading | pubmed-meshheading:1725262-... | lld:pubmed |
| pubmed-article:1725262 | pubmed:meshHeading | pubmed-meshheading:1725262-... | lld:pubmed |
| pubmed-article:1725262 | pubmed:meshHeading | pubmed-meshheading:1725262-... | lld:pubmed |
| pubmed-article:1725262 | pubmed:meshHeading | pubmed-meshheading:1725262-... | lld:pubmed |
| pubmed-article:1725262 | pubmed:year | 1991 | lld:pubmed |
| pubmed-article:1725262 | pubmed:articleTitle | Mutant enzymes and tRNAs as probes of the glutaminyl-tRNA synthetase: tRNA(Gln) interaction. | lld:pubmed |
| pubmed-article:1725262 | pubmed:affiliation | Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06511. | lld:pubmed |
| pubmed-article:1725262 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1725262 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:1725262 | pubmed:publicationType | Review | lld:pubmed |
| entrez-gene:945310 | entrezgene:pubmed | pubmed-article:1725262 | lld:entrezgene |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1725262 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1725262 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1725262 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1725262 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1725262 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1725262 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1725262 | lld:pubmed |
