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pubmed-article:1724721pubmed:abstractTextThe paper describes a sensitive latex hybridization assay (LHA) method applied for indirect detection of biotinylated nucleic acid hybrids immobilized on a synthetic membrane. The biotinylated hybrids were visualized by means of latex particles containing the fluorescent dye pyronine G and coated with streptavidin; 1.6 and 0.3 pg of lambda-phage DNA was detected by dot blot hybridizations on nylon membrane and polyethyleneimine-cellophane, respectively. The assay sensitivity was increased by three orders of magnitude over that with fluorescently labeled probes due to encapsulation of the fluorescent dye in polymer particles. LHA is simple (single-stage detection procedure), fast, and more sensitive than any of the other nonradioactive hybridization methods.lld:pubmed
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pubmed-article:1724721pubmed:articleTitleA novel approach to nonradioactive hybridization assay of nucleic acids using stained latex particles.lld:pubmed
pubmed-article:1724721pubmed:affiliationShemyakin Institute of Bioorganic Chemistry, USSR Academy of Sciences, Moscow.lld:pubmed
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