pubmed-article:17240450 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:17240450 | lifeskim:mentions | umls-concept:C0079904 | lld:lifeskim |
pubmed-article:17240450 | lifeskim:mentions | umls-concept:C0663914 | lld:lifeskim |
pubmed-article:17240450 | lifeskim:mentions | umls-concept:C0021467 | lld:lifeskim |
pubmed-article:17240450 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
pubmed-article:17240450 | lifeskim:mentions | umls-concept:C1523116 | lld:lifeskim |
pubmed-article:17240450 | lifeskim:mentions | umls-concept:C0021469 | lld:lifeskim |
pubmed-article:17240450 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
pubmed-article:17240450 | lifeskim:mentions | umls-concept:C1879547 | lld:lifeskim |
pubmed-article:17240450 | lifeskim:mentions | umls-concept:C0065538 | lld:lifeskim |
pubmed-article:17240450 | pubmed:issue | 10 | lld:pubmed |
pubmed-article:17240450 | pubmed:dateCreated | 2007-2-19 | lld:pubmed |
pubmed-article:17240450 | pubmed:abstractText | The mis-regulation of nuclear factor-kappa B (NF-kappaB) signal pathway is involved in a variety of inflammatory diseases that leds to the production of inflammatory mediators. Our studies using human U937 promonocytes cells suggested that magnolol, a low molecular weight lignan isolated from the medicinal plant Magnolia officinalis, differentially down-regulated the pharmacologically induced expression of NF-kappaB-regulated inflammatory gene products MMP-9, IL-8, MCP-1, MIP-1alpha, TNF-alpha. Pre-treatment of magnolol blocked TNF-alpha-induced NF-kappaB activation in different cell types as evidenced by EMSA. Magnolol did not directly affect the binding of p65/p50 heterodimer to DNA. Immunoblot analysis demonstrated that magnolol inhibited the TNF-alpha-stimulated phosphorylation and degradation of the cytosolic NF-kappaB inhibitor IkappaBalpha and the effects were dose-dependent. Mechanistically, a non-radioactive IkappaB kinases (IKK) assay using immunoprecipitated IKKs protein demonstrated that magnolol inhibited both intrinsic and TNF-alpha-stimulated IKK activity, thus suggesting a critical role of magnolol in abrogating the phosphorylation and degradation of IkappaBalpha. The involvement of IKK was further verified in a HeLa cell NF-kappaB-dependent luciferase reporter system. In this system magnolol suppressed luciferase expression stimulated by TNF-alpha and by the transient transfection and expression of NIK (NF-kappaB-inducing kinase), wild type IKKbeta, constitutively active IKKalpha and IKKbeta, or the p65 subunit. Magnolol was also found to inhibit the nuclear translocation and phosphorylation of p65 subunit of NF-kappaB. In line with the observation that NF-kappaB activation may up-regulate anti-apoptotic genes, it was shown in U937 cells that magnolol enhanced TNF-alpha-induced apoptotic cell death. Our results suggest that magnolol or its derivatives may have potential anti-inflammatory actions through IKK inactivation. | lld:pubmed |
pubmed-article:17240450 | pubmed:language | eng | lld:pubmed |
pubmed-article:17240450 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:17240450 | pubmed:citationSubset | IM | lld:pubmed |
pubmed-article:17240450 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:17240450 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:17240450 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:17240450 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:17240450 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:17240450 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:17240450 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:17240450 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:17240450 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:17240450 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:17240450 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:17240450 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:17240450 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:17240450 | pubmed:month | Apr | lld:pubmed |
pubmed-article:17240450 | pubmed:issn | 0161-5890 | lld:pubmed |
pubmed-article:17240450 | pubmed:author | pubmed-author:YangMengsuM | lld:pubmed |
pubmed-article:17240450 | pubmed:author | pubmed-author:FongWang-FunW... | lld:pubmed |
pubmed-article:17240450 | pubmed:author | pubmed-author:CheungHon-Yeu... | lld:pubmed |
pubmed-article:17240450 | pubmed:author | pubmed-author:ShenXiao-Ling... | lld:pubmed |
pubmed-article:17240450 | pubmed:author | pubmed-author:TseAnfernee... | lld:pubmed |
pubmed-article:17240450 | pubmed:author | pubmed-author:WanChi-KeungC... | lld:pubmed |
pubmed-article:17240450 | pubmed:author | pubmed-author:ZhuGuo-YuanGY | lld:pubmed |
pubmed-article:17240450 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:17240450 | pubmed:volume | 44 | lld:pubmed |
pubmed-article:17240450 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:17240450 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:17240450 | pubmed:pagination | 2647-58 | lld:pubmed |
pubmed-article:17240450 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
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pubmed-article:17240450 | pubmed:year | 2007 | lld:pubmed |
pubmed-article:17240450 | pubmed:articleTitle | Magnolol suppresses NF-kappaB activation and NF-kappaB regulated gene expression through inhibition of IkappaB kinase activation. | lld:pubmed |
pubmed-article:17240450 | pubmed:affiliation | Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon Tong, Hong Kong SAR, China. | lld:pubmed |
pubmed-article:17240450 | pubmed:publicationType | Journal Article | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17240450 | lld:pubmed |