| pubmed-article:17222422 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:17222422 | lifeskim:mentions | umls-concept:C0025663 | lld:lifeskim |
| pubmed-article:17222422 | lifeskim:mentions | umls-concept:C0021756 | lld:lifeskim |
| pubmed-article:17222422 | lifeskim:mentions | umls-concept:C0009393 | lld:lifeskim |
| pubmed-article:17222422 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:17222422 | lifeskim:mentions | umls-concept:C0301872 | lld:lifeskim |
| pubmed-article:17222422 | lifeskim:mentions | umls-concept:C0920508 | lld:lifeskim |
| pubmed-article:17222422 | lifeskim:mentions | umls-concept:C1554184 | lld:lifeskim |
| pubmed-article:17222422 | lifeskim:mentions | umls-concept:C0521115 | lld:lifeskim |
| pubmed-article:17222422 | pubmed:issue | 1-2 | lld:pubmed |
| pubmed-article:17222422 | pubmed:dateCreated | 2007-3-12 | lld:pubmed |
| pubmed-article:17222422 | pubmed:abstractText | The single color IFN-gamma ELISPOT assay has become a standard for assessing HIV-specific immune responses in HIV-infected subjects. However, recent data suggests that single cytokine detection for immune monitoring of HIV-infected individuals may not be sufficient to fully describe virus-specific immune responses. Here, we have designed and validated a dual color ELISPOT assay capable of detecting both IL-2 and IFN-gamma secreting cells simultaneously in response to HIV antigens. We found that a cell input number of 200,000 cells/well provided a good balance between limited availability of cells due to blood volume restrictions and ability to detect all cytokine secretion patterns. The simultaneous detection of IL-2 and IFN-gamma resulted in a decreased magnitude of IFN-gamma but not IL-2 responses. Measures of intra- and inter-assay variability for the dual color ELISPOT assay were comparable to that seen for single cytokine ELISPOT assay with coefficients of variation below 20% for IL-2, IFN-gamma and dual secretion. Although CD8+ T cells mediated most HIV-specific responses in infected subjects, CD4+ T cells mediated responses to HIV were also detected. Features of this assay such as high throughput, cell number requirement and cytokine choice should make this assay a valuable tool for screening for HIV-specific immune responses in several clinically relevant settings. | lld:pubmed |
| pubmed-article:17222422 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17222422 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17222422 | pubmed:language | eng | lld:pubmed |
| pubmed-article:17222422 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17222422 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:17222422 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17222422 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17222422 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17222422 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:17222422 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:17222422 | pubmed:issn | 0022-1759 | lld:pubmed |
| pubmed-article:17222422 | pubmed:author | pubmed-author:RoutyJean-Pie... | lld:pubmed |
| pubmed-article:17222422 | pubmed:author | pubmed-author:BernardNicole... | lld:pubmed |
| pubmed-article:17222422 | pubmed:author | pubmed-author:PeretzYoavY | lld:pubmed |
| pubmed-article:17222422 | pubmed:author | pubmed-author:SekalyRafick-... | lld:pubmed |
| pubmed-article:17222422 | pubmed:author | pubmed-author:BoulasselMoha... | lld:pubmed |
| pubmed-article:17222422 | pubmed:author | pubmed-author:TremblayCecil... | lld:pubmed |
| pubmed-article:17222422 | pubmed:author | pubmed-author:BoisvertMarie... | lld:pubmed |
| pubmed-article:17222422 | pubmed:author | pubmed-author:BouletSalixS | lld:pubmed |
| pubmed-article:17222422 | pubmed:author | pubmed-author:NdongalaMiche... | lld:pubmed |
| pubmed-article:17222422 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:17222422 | pubmed:day | 30 | lld:pubmed |
| pubmed-article:17222422 | pubmed:volume | 320 | lld:pubmed |
| pubmed-article:17222422 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:17222422 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:17222422 | pubmed:pagination | 18-29 | lld:pubmed |
| pubmed-article:17222422 | pubmed:dateRevised | 2011-10-17 | lld:pubmed |
| pubmed-article:17222422 | pubmed:meshHeading | pubmed-meshheading:17222422... | lld:pubmed |
| pubmed-article:17222422 | pubmed:meshHeading | pubmed-meshheading:17222422... | lld:pubmed |
| pubmed-article:17222422 | pubmed:meshHeading | pubmed-meshheading:17222422... | lld:pubmed |
| pubmed-article:17222422 | pubmed:meshHeading | pubmed-meshheading:17222422... | lld:pubmed |
| pubmed-article:17222422 | pubmed:meshHeading | pubmed-meshheading:17222422... | lld:pubmed |
| pubmed-article:17222422 | pubmed:meshHeading | pubmed-meshheading:17222422... | lld:pubmed |
| pubmed-article:17222422 | pubmed:meshHeading | pubmed-meshheading:17222422... | lld:pubmed |
| pubmed-article:17222422 | pubmed:meshHeading | pubmed-meshheading:17222422... | lld:pubmed |
| pubmed-article:17222422 | pubmed:year | 2007 | lld:pubmed |
| pubmed-article:17222422 | pubmed:articleTitle | A dual color ELISPOT method for the simultaneous detection of IL-2 and IFN-gamma HIV-specific immune responses. | lld:pubmed |
| pubmed-article:17222422 | pubmed:affiliation | Research Institute of the McGill University Health Center, Montreal, Canada. | lld:pubmed |
| pubmed-article:17222422 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:17222422 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:17222422 | pubmed:publicationType | Evaluation Studies | lld:pubmed |
| pubmed-article:17222422 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17222422 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17222422 | lld:pubmed |
