Linked Life Data

17220308

Source:http://linkedlifedata.com/resource/pubmed/id/17220308

Statements in which the resource exists.
SubjectPredicateObjectContext
pubmed-article:17220308rdf:typepubmed:Citationlld:pubmed
pubmed-article:17220308lifeskim:mentionsumls-concept:C0033809lld:lifeskim
pubmed-article:17220308lifeskim:mentionsumls-concept:C0085080lld:lifeskim
pubmed-article:17220308lifeskim:mentionsumls-concept:C0031308lld:lifeskim
pubmed-article:17220308lifeskim:mentionsumls-concept:C1171362lld:lifeskim
pubmed-article:17220308lifeskim:mentionsumls-concept:C0205419lld:lifeskim
pubmed-article:17220308lifeskim:mentionsumls-concept:C0017262lld:lifeskim
pubmed-article:17220308lifeskim:mentionsumls-concept:C1420003lld:lifeskim
pubmed-article:17220308lifeskim:mentionsumls-concept:C1420002lld:lifeskim
pubmed-article:17220308lifeskim:mentionsumls-concept:C1515670lld:lifeskim
pubmed-article:17220308pubmed:issue3lld:pubmed
pubmed-article:17220308pubmed:dateCreated2007-2-22lld:pubmed
pubmed-article:17220308pubmed:abstractTextSurfactant protein A (SP-A) enhances phagocytosis of Pseudomonas aeruginosa. Two functional genes, SP-A1 and SP-A2, encode human SP-A. As we showed before, baculovirus-mediated insect cell-expressed SP-A2 enhances the association of P. aeruginosa with rat alveolar macrophages (rAMs) more than does SP-A1. However, true phagocytosis (internalization) was not shown, and insect cell derived proteins lack or are defective in certain mammalian posttranslational modifications that may be important for SP-A1 and SP-A2 activity and specificity. Here we used SP-A1 (6A(2), 6A(4)) and SP-A2 (1A(0), 1A(1)) allele variants expressed by CHO (Chinese hamster ovary) mammalian cells to study their effect on association and/or internalization of P. aeruginosa by rAMs and/or human AMs (hAMs) and to study if phagocytosis can be modulated differentially and/or more effectively by CHO cell-expressed SP-A variants than by insect-cell expressed SP-A variants. For cell association and internalization assessments, light microscopy and fluorescence-activated cell sorter analyses were used, respectively. We found the following for the first time. (i) SP-A2 variants enhanced phagocytosis (cell association and/or internalization) of P. aeruginosa more than SP-A1 variants did, and the cell association correlated with internalization. (ii) Differences in the activities of SP-A variants were observed in the following order: 1A(1)>1A(0)>6A(2)>6A(4). (iii) rAMs, although more active than hAMs, are an appropriate model, as SP-A2 variants exhibited activity higher than that seen for SP-A1 variants with either rAMs or hAMs. (iv) CHO cell-expressed SP-A was considerably more active than insect cell-expressed variants. We conclude that SP-A2 variants stimulate phagocytosis of P. aeruginosa more effectively than SP-A1 variants and that posttranslational modifications positively influence the phagocytic activity of SP-A.lld:pubmed
pubmed-article:17220308pubmed:granthttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:languageenglld:pubmed
pubmed-article:17220308pubmed:journalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:citationSubsetIMlld:pubmed
pubmed-article:17220308pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:17220308pubmed:statusMEDLINElld:pubmed
pubmed-article:17220308pubmed:monthMarlld:pubmed
pubmed-article:17220308pubmed:issn0019-9567lld:pubmed
pubmed-article:17220308pubmed:authorpubmed-author:WangGuirongGlld:pubmed
pubmed-article:17220308pubmed:authorpubmed-author:UmsteadTodd...lld:pubmed
pubmed-article:17220308pubmed:authorpubmed-author:PhelpsDavid...lld:pubmed
pubmed-article:17220308pubmed:authorpubmed-author:FlorosJoannaJlld:pubmed
pubmed-article:17220308pubmed:authorpubmed-author:ThomasNeal...lld:pubmed
pubmed-article:17220308pubmed:authorpubmed-author:ZacharatosMar...lld:pubmed
pubmed-article:17220308pubmed:authorpubmed-author:MikerovAnatol...lld:pubmed
pubmed-article:17220308pubmed:issnTypePrintlld:pubmed
pubmed-article:17220308pubmed:volume75lld:pubmed
pubmed-article:17220308pubmed:ownerNLMlld:pubmed
pubmed-article:17220308pubmed:authorsCompleteYlld:pubmed
pubmed-article:17220308pubmed:pagination1403-12lld:pubmed
pubmed-article:17220308pubmed:dateRevised2009-11-18lld:pubmed
pubmed-article:17220308pubmed:meshHeadingpubmed-meshheading:17220308...lld:pubmed
pubmed-article:17220308pubmed:meshHeadingpubmed-meshheading:17220308...lld:pubmed
pubmed-article:17220308pubmed:meshHeadingpubmed-meshheading:17220308...lld:pubmed
pubmed-article:17220308pubmed:meshHeadingpubmed-meshheading:17220308...lld:pubmed
pubmed-article:17220308pubmed:meshHeadingpubmed-meshheading:17220308...lld:pubmed
pubmed-article:17220308pubmed:meshHeadingpubmed-meshheading:17220308...lld:pubmed
pubmed-article:17220308pubmed:meshHeadingpubmed-meshheading:17220308...lld:pubmed
pubmed-article:17220308pubmed:meshHeadingpubmed-meshheading:17220308...lld:pubmed
pubmed-article:17220308pubmed:meshHeadingpubmed-meshheading:17220308...lld:pubmed
pubmed-article:17220308pubmed:meshHeadingpubmed-meshheading:17220308...lld:pubmed
More...