| pubmed-article:17209005 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:17209005 | lifeskim:mentions | umls-concept:C0206745 | lld:lifeskim |
| pubmed-article:17209005 | lifeskim:mentions | umls-concept:C0344432 | lld:lifeskim |
| pubmed-article:17209005 | lifeskim:mentions | umls-concept:C0065170 | lld:lifeskim |
| pubmed-article:17209005 | lifeskim:mentions | umls-concept:C0205161 | lld:lifeskim |
| pubmed-article:17209005 | lifeskim:mentions | umls-concept:C1832073 | lld:lifeskim |
| pubmed-article:17209005 | lifeskim:mentions | umls-concept:C0596235 | lld:lifeskim |
| pubmed-article:17209005 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:17209005 | pubmed:dateCreated | 2007-5-9 | lld:pubmed |
| pubmed-article:17209005 | pubmed:abstractText | Patients with mutations in the mitochondrial very-long-chain acyl-CoA dehydrogenase (VLCAD) gene are at risk for cardiomyopathy, myocardial dysfunction, ventricular tachycardia (VT), and sudden cardiac death. The mechanism is not known. Here we report a novel mechanism of VT in mice lacking VLCAD (VLCAD(-/-)). These mice exhibited polymorphic VT and increased incidence of VT after isoproterenol infusion. Polymorphic VT was induced in 10 out of 12 VLCAD(-/-) mice (83%) when isoproterenol was used. One out of 10 VLCAD(-/-) mice with polymorphic VT had VT with the typical bidirectional morphology. At the molecular level, VLCAD(-/-) cardiomyocytes showed increased levels of cardiac ryanodine receptor 2, phospholamban, and calsequestrin with increased [(3)H]ryanodine binding in heart microsomes. At the single cardiomyocyte level, VLCAD(-/-) cardiomyocytes showed significant increase in diastolic indo 1 and fura 2 fluorescence, with increased Ca(2+) transient amplitude. These changes were associated with altered Ca(2+) dynamics, to include: faster sarcomere contraction, larger time derivative of the upstroke, and shorter time-to-minimum sarcomere length compared with VLCAD(+/+) control cells. The L-type Ca(2+) current characteristics were not different under voltage-clamp conditions in the two VLCAD genotypes. Sarcoplasmic reticulum Ca(2+) load measured as normalized integrated Na(+)/Ca(2+) exchange current after rapid caffeine application was increased by 48% in VLCAD(-/-) cells. We conclude that intracellular Ca(2+) handling represents a possible molecular mechanism of arrhythmias in mice and perhaps in VLCAD-deficient humans. | lld:pubmed |
| pubmed-article:17209005 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17209005 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17209005 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17209005 | pubmed:language | eng | lld:pubmed |
| pubmed-article:17209005 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17209005 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:17209005 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17209005 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17209005 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:17209005 | pubmed:month | May | lld:pubmed |
| pubmed-article:17209005 | pubmed:issn | 0363-6135 | lld:pubmed |
| pubmed-article:17209005 | pubmed:author | pubmed-author:AndersonMark... | lld:pubmed |
| pubmed-article:17209005 | pubmed:author | pubmed-author:MilatovicDeja... | lld:pubmed |
| pubmed-article:17209005 | pubmed:author | pubmed-author:AschnerMichae... | lld:pubmed |
| pubmed-article:17209005 | pubmed:author | pubmed-author:JeyakumarLoic... | lld:pubmed |
| pubmed-article:17209005 | pubmed:author | pubmed-author:FleischerSidn... | lld:pubmed |
| pubmed-article:17209005 | pubmed:author | pubmed-author:KannankerilPr... | lld:pubmed |
| pubmed-article:17209005 | pubmed:author | pubmed-author:DzhuraIgorI | lld:pubmed |
| pubmed-article:17209005 | pubmed:author | pubmed-author:StraussArnold... | lld:pubmed |
| pubmed-article:17209005 | pubmed:author | pubmed-author:ExilVernat... | lld:pubmed |
| pubmed-article:17209005 | pubmed:author | pubmed-author:BaudenbacherF... | lld:pubmed |
| pubmed-article:17209005 | pubmed:author | pubmed-author:WerdichAndrea... | lld:pubmed |
| pubmed-article:17209005 | pubmed:author | pubmed-author:LeGroneAlison... | lld:pubmed |
| pubmed-article:17209005 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:17209005 | pubmed:volume | 292 | lld:pubmed |
| pubmed-article:17209005 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:17209005 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:17209005 | pubmed:pagination | H2202-11 | lld:pubmed |
| pubmed-article:17209005 | pubmed:dateRevised | 2007-12-3 | lld:pubmed |
| pubmed-article:17209005 | pubmed:meshHeading | pubmed-meshheading:17209005... | lld:pubmed |
| pubmed-article:17209005 | pubmed:meshHeading | pubmed-meshheading:17209005... | lld:pubmed |
| pubmed-article:17209005 | pubmed:meshHeading | pubmed-meshheading:17209005... | lld:pubmed |
| pubmed-article:17209005 | pubmed:meshHeading | pubmed-meshheading:17209005... | lld:pubmed |
| pubmed-article:17209005 | pubmed:meshHeading | pubmed-meshheading:17209005... | lld:pubmed |
| pubmed-article:17209005 | pubmed:meshHeading | pubmed-meshheading:17209005... | lld:pubmed |
| pubmed-article:17209005 | pubmed:meshHeading | pubmed-meshheading:17209005... | lld:pubmed |
| pubmed-article:17209005 | pubmed:meshHeading | pubmed-meshheading:17209005... | lld:pubmed |
| pubmed-article:17209005 | pubmed:meshHeading | pubmed-meshheading:17209005... | lld:pubmed |
| pubmed-article:17209005 | pubmed:meshHeading | pubmed-meshheading:17209005... | lld:pubmed |
| pubmed-article:17209005 | pubmed:year | 2007 | lld:pubmed |
| pubmed-article:17209005 | pubmed:articleTitle | Polymorphic ventricular tachycardia and abnormal Ca2+ handling in very-long-chain acyl-CoA dehydrogenase null mice. | lld:pubmed |
| pubmed-article:17209005 | pubmed:affiliation | Division of Cardiology, Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, TN 37232-0001, USA. | lld:pubmed |
| pubmed-article:17209005 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:17209005 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:17209005 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
| entrez-gene:11370 | entrezgene:pubmed | pubmed-article:17209005 | lld:entrezgene |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17209005 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17209005 | lld:pubmed |
