| pubmed-article:17202488 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:17202488 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:17202488 | lifeskim:mentions | umls-concept:C0015283 | lld:lifeskim |
| pubmed-article:17202488 | lifeskim:mentions | umls-concept:C0682972 | lld:lifeskim |
| pubmed-article:17202488 | lifeskim:mentions | umls-concept:C1427949 | lld:lifeskim |
| pubmed-article:17202488 | lifeskim:mentions | umls-concept:C0031671 | lld:lifeskim |
| pubmed-article:17202488 | lifeskim:mentions | umls-concept:C0279023 | lld:lifeskim |
| pubmed-article:17202488 | lifeskim:mentions | umls-concept:C1546857 | lld:lifeskim |
| pubmed-article:17202488 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:17202488 | pubmed:dateCreated | 2007-1-4 | lld:pubmed |
| pubmed-article:17202488 | pubmed:abstractText | The vesicle priming protein Munc13-1 is regulated by diacylglycerol (DAG) and is therefore hypothesized to play a role in the control of neurotransmitter release by phospholipase C (PLC)-coupled receptors. We combined voltage-clamp recordings of voltage-gated Ca2+ channels (VGCCs) and high-resolution capacitance measurements to investigate the mechanism of receptor-mediated modulation of exocytosis in bovine chromaffin cells. Activation of endogenous H1 G(q)-protein-coupled receptors (G(q)PCRs) by histamine potentiated stimulus-coupled secretion despite concurrently inhibiting Ca2+ influx through VGCCs. Histamine increased the size of the readily releasable pool of vesicles and in particular a subpool of fusion-competent vesicles localized in close proximity to VGCCs. Pharmacological characterization showed that potentiation of exocytosis depended on the activation of PLC but not protein kinase C. Overexpression of wild-type Munc13-1 by adenoviral infection had no effect on histamine-induced potentiation per se, whereas DAG-insensitive Munc13-1(H567K) completely abolished it. This is the first endogenous mammalian G(q)PCR signaling pathway identified that engages Munc13-1 to increase stimulus-coupled secretion by recruiting vesicles to the immediately releasable pool. G(q)PCRs are therefore able to control exocytosis at the level of SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) complex formation to produce rapid, short-term potentiation of the secretory output of neurons and endocrine cells. | lld:pubmed |
| pubmed-article:17202488 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17202488 | pubmed:language | eng | lld:pubmed |
| pubmed-article:17202488 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17202488 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:17202488 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17202488 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17202488 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17202488 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17202488 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17202488 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:17202488 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:17202488 | pubmed:issn | 1529-2401 | lld:pubmed |
| pubmed-article:17202488 | pubmed:author | pubmed-author:WoolleyRobert... | lld:pubmed |
| pubmed-article:17202488 | pubmed:author | pubmed-author:BauerClaudia... | lld:pubmed |
| pubmed-article:17202488 | pubmed:author | pubmed-author:SewardElizabe... | lld:pubmed |
| pubmed-article:17202488 | pubmed:author | pubmed-author:TeschemacherA... | lld:pubmed |
| pubmed-article:17202488 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:17202488 | pubmed:day | 3 | lld:pubmed |
| pubmed-article:17202488 | pubmed:volume | 27 | lld:pubmed |
| pubmed-article:17202488 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:17202488 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:17202488 | pubmed:pagination | 212-9 | lld:pubmed |
| pubmed-article:17202488 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
| pubmed-article:17202488 | pubmed:meshHeading | pubmed-meshheading:17202488... | lld:pubmed |
| pubmed-article:17202488 | pubmed:meshHeading | pubmed-meshheading:17202488... | lld:pubmed |
| pubmed-article:17202488 | pubmed:meshHeading | pubmed-meshheading:17202488... | lld:pubmed |
| pubmed-article:17202488 | pubmed:meshHeading | pubmed-meshheading:17202488... | lld:pubmed |
| pubmed-article:17202488 | pubmed:meshHeading | pubmed-meshheading:17202488... | lld:pubmed |
| pubmed-article:17202488 | pubmed:meshHeading | pubmed-meshheading:17202488... | lld:pubmed |
| pubmed-article:17202488 | pubmed:meshHeading | pubmed-meshheading:17202488... | lld:pubmed |
| pubmed-article:17202488 | pubmed:meshHeading | pubmed-meshheading:17202488... | lld:pubmed |
| pubmed-article:17202488 | pubmed:meshHeading | pubmed-meshheading:17202488... | lld:pubmed |
| pubmed-article:17202488 | pubmed:meshHeading | pubmed-meshheading:17202488... | lld:pubmed |
| pubmed-article:17202488 | pubmed:year | 2007 | lld:pubmed |
| pubmed-article:17202488 | pubmed:articleTitle | Potentiation of exocytosis by phospholipase C-coupled G-protein-coupled receptors requires the priming protein Munc13-1. | lld:pubmed |
| pubmed-article:17202488 | pubmed:affiliation | Department of Biomedical Science, University of Sheffield, Sheffield S10 2TN, United Kingdom. | lld:pubmed |
| pubmed-article:17202488 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:17202488 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17202488 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17202488 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17202488 | lld:pubmed |
