| pubmed-article:17194156 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:17194156 | lifeskim:mentions | umls-concept:C0178539 | lld:lifeskim |
| pubmed-article:17194156 | lifeskim:mentions | umls-concept:C0012854 | lld:lifeskim |
| pubmed-article:17194156 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:17194156 | lifeskim:mentions | umls-concept:C0205195 | lld:lifeskim |
| pubmed-article:17194156 | lifeskim:mentions | umls-concept:C0061279 | lld:lifeskim |
| pubmed-article:17194156 | lifeskim:mentions | umls-concept:C0049196 | lld:lifeskim |
| pubmed-article:17194156 | lifeskim:mentions | umls-concept:C0332324 | lld:lifeskim |
| pubmed-article:17194156 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:17194156 | pubmed:dateCreated | 2006-12-29 | lld:pubmed |
| pubmed-article:17194156 | pubmed:abstractText | Nucleoside 5-formyl-2'-deoxyuridine (FodU) is a major thymidine lesion generated by reactive oxygen species. In vitro and in vivo replication studies revealed that FodU can be mutagenic. A reliable and sensitive quantification method is, therefore, important for assessing the biological implications of this lesion. However, the detection limit of FodU by liquid chromatography-tandem mass spectrometry (LC-MS/MS) was relatively poor compared with those of other oxidative DNA base damages. In this paper we described a new approach for the highly sensitive detection of FodU. We derivatized FodU with Girard reagent T to form a hydrazone conjugate harboring a precharged quaternary ammonium moiety, which enabled the facile detection of the resulting conjugate by positive-ion electrospray ionization MS. We also showed that the combination of derivatization with LC-MS/MS on a linear-ion-trap mass spectrometer could allow for the quantification of FodU at a detection limit of 3-4 fmol, which is approximately 20-fold better than that for the direct analysis of the underivatized compound. By using isotope-labeled FodU as the internal standard and this derivatization method, we further quantified, by using LC-MS/MS, the yield of FodU formed in cellular DNA. | lld:pubmed |
| pubmed-article:17194156 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17194156 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17194156 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17194156 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17194156 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17194156 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17194156 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17194156 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17194156 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17194156 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17194156 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17194156 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17194156 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17194156 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17194156 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17194156 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17194156 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17194156 | pubmed:language | eng | lld:pubmed |
| pubmed-article:17194156 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17194156 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:17194156 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17194156 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17194156 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17194156 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17194156 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17194156 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17194156 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17194156 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:17194156 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:17194156 | pubmed:issn | 0003-2700 | lld:pubmed |
| pubmed-article:17194156 | pubmed:author | pubmed-author:WangYinshengY | lld:pubmed |
| pubmed-article:17194156 | pubmed:author | pubmed-author:HongHaizhengH | lld:pubmed |
| pubmed-article:17194156 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:17194156 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:17194156 | pubmed:volume | 79 | lld:pubmed |
| pubmed-article:17194156 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:17194156 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:17194156 | pubmed:pagination | 322-6 | lld:pubmed |
| pubmed-article:17194156 | pubmed:dateRevised | 2011-5-3 | lld:pubmed |
| pubmed-article:17194156 | pubmed:meshHeading | pubmed-meshheading:17194156... | lld:pubmed |
| pubmed-article:17194156 | pubmed:meshHeading | pubmed-meshheading:17194156... | lld:pubmed |
| pubmed-article:17194156 | pubmed:meshHeading | pubmed-meshheading:17194156... | lld:pubmed |
| pubmed-article:17194156 | pubmed:meshHeading | pubmed-meshheading:17194156... | lld:pubmed |
| pubmed-article:17194156 | pubmed:meshHeading | pubmed-meshheading:17194156... | lld:pubmed |
| pubmed-article:17194156 | pubmed:meshHeading | pubmed-meshheading:17194156... | lld:pubmed |
| pubmed-article:17194156 | pubmed:meshHeading | pubmed-meshheading:17194156... | lld:pubmed |
| pubmed-article:17194156 | pubmed:meshHeading | pubmed-meshheading:17194156... | lld:pubmed |
| pubmed-article:17194156 | pubmed:meshHeading | pubmed-meshheading:17194156... | lld:pubmed |
| pubmed-article:17194156 | pubmed:meshHeading | pubmed-meshheading:17194156... | lld:pubmed |
| pubmed-article:17194156 | pubmed:meshHeading | pubmed-meshheading:17194156... | lld:pubmed |
| pubmed-article:17194156 | pubmed:meshHeading | pubmed-meshheading:17194156... | lld:pubmed |
| pubmed-article:17194156 | pubmed:year | 2007 | lld:pubmed |
| pubmed-article:17194156 | pubmed:articleTitle | Derivatization with Girard reagent T combined with LC-MS/MS for the sensitive detection of 5-formyl-2'-deoxyuridine in cellular DNA. | lld:pubmed |
| pubmed-article:17194156 | pubmed:affiliation | Environmental Toxicology Graduate Program and Department of Chemistry, University of California at Riverside, Riverside, California 92521, USA. | lld:pubmed |
| pubmed-article:17194156 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:17194156 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17194156 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17194156 | lld:pubmed |
