Linked Life Data

17192852

Source:http://linkedlifedata.com/resource/pubmed/id/17192852

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pubmed-article:17192852pubmed:abstractTextThe fermentation of maltotriose, the second most abundant fermentable sugar in wort, is often incomplete during high-gravity brewing. Poor maltotriose consumption is due to environmental stress conditions during high-gravity fermentation and especially to a low uptake of this sugar by some industrial strains. In this study we investigated whether the use of strains with an alpha-glucosidase attached to the outside of the cell might be a possible way to reduce residual maltotriose. To this end, the N-terminal leader sequence of Kre1 and the carboxy-terminal anchoring domain of either Cwp2 or Flo1 were used to target maltase encoded by MAL32 to the cell surface. We showed that Mal32 displayed on the cell surface of Saccharomyces cerevisiae laboratory strains was capable of hydrolysis of alpha-1,4-linkages, and that it increased the ability of a strain lacking a functional maltose permease to grow on maltotriose. Moreover, the enzyme was also expressed and found to be active in an industrial strain. These data show that expressing a suitable maltase on the cell surface might provide a means of modifying yeast for more complete maltotriose utilization in brewing and other fermentation applications.lld:pubmed
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pubmed-article:17192852pubmed:issn0749-503Xlld:pubmed
pubmed-article:17192852pubmed:authorpubmed-author:BrandtRRlld:pubmed
pubmed-article:17192852pubmed:authorpubmed-author:SteensmaH YHYlld:pubmed
pubmed-article:17192852pubmed:authorpubmed-author:Van DijckPPlld:pubmed
pubmed-article:17192852pubmed:authorpubmed-author:DietvorstJJlld:pubmed
pubmed-article:17192852pubmed:authorpubmed-author:BlieckLLlld:pubmed
pubmed-article:17192852pubmed:copyrightInfoCopyright (c) 2007 John Wiley & Sons, Ltd.lld:pubmed
pubmed-article:17192852pubmed:issnTypePrintlld:pubmed
pubmed-article:17192852pubmed:volume24lld:pubmed
pubmed-article:17192852pubmed:ownerNLMlld:pubmed
pubmed-article:17192852pubmed:authorsCompleteYlld:pubmed
pubmed-article:17192852pubmed:pagination27-38lld:pubmed
pubmed-article:17192852pubmed:dateRevised2009-11-19lld:pubmed
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pubmed-article:17192852pubmed:year2007lld:pubmed
pubmed-article:17192852pubmed:articleTitleAttachment of MAL32-encoded maltase on the outside of yeast cells improves maltotriose utilization.lld:pubmed
pubmed-article:17192852pubmed:affiliationInstitute of Biology, Leiden University, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands.lld:pubmed
pubmed-article:17192852pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:17192852pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed