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pubmed-article:17191866pubmed:abstractTextSpectroscopic properties of single-stranded DNA/schizophyllan ternary complexes (ss-DNA2s-SPG), induced by addition of either complementary or noncomplementary strands, have been investigated. The addition of the complementary strands to ss-DNA2s-SPG induced the quick release of the bound ss-DNA to the complementary strands (both DNA and RNA), whereas the ternary complex was unaffected upon addition of noncomplementary strands. Our experiments imply that SPG has complexation properties indispensable to the gene carriers. As far as we know, there is no report on exploitation of such nonviral gene carriers that can accomplish an intelligent release of the bound ss-DNA toward the complementary strands. We believe, therefore, that SPG, a natural and neutral polysaccharide, has a great potential to become a new ss-DNA carrier.lld:pubmed
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pubmed-article:17191866pubmed:authorpubmed-author:ShinkaiSeijiSlld:pubmed
pubmed-article:17191866pubmed:authorpubmed-author:KoumotoKazuya...lld:pubmed
pubmed-article:17191866pubmed:authorpubmed-author:SakuraiKazuoKlld:pubmed
pubmed-article:17191866pubmed:authorpubmed-author:MizuMasamiMlld:pubmed
pubmed-article:17191866pubmed:authorpubmed-author:KunitakeToyok...lld:pubmed
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pubmed-article:17191866pubmed:pagination520-9lld:pubmed
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pubmed-article:17191866pubmed:articleTitlePolysaccharide/polynucleotide complexes. Part 6: complementary-strand-induced release of single-stranded DNA bound in the schizophyllan complex.lld:pubmed
pubmed-article:17191866pubmed:affiliationDepartment of Chemical Processes and Environments, Faculty of Environmental Engineering, The University of Kitakyushu, 1-1 Hibikino, Wakamatsu-ku, Kitakyushu, Fukuoka 808-0135, Japan.lld:pubmed
pubmed-article:17191866pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:17191866pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed