| pubmed-article:17178797 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:17178797 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:17178797 | lifeskim:mentions | umls-concept:C0004037 | lld:lifeskim |
| pubmed-article:17178797 | lifeskim:mentions | umls-concept:C0026473 | lld:lifeskim |
| pubmed-article:17178797 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:17178797 | lifeskim:mentions | umls-concept:C0393080 | lld:lifeskim |
| pubmed-article:17178797 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:17178797 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:17178797 | lifeskim:mentions | umls-concept:C0150312 | lld:lifeskim |
| pubmed-article:17178797 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:17178797 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:17178797 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:17178797 | pubmed:dateCreated | 2007-2-22 | lld:pubmed |
| pubmed-article:17178797 | pubmed:abstractText | We assessed the effect of voriconazole (VRC) on the expression and release of selected cytokines and chemokines in the THP-1 human monocytic cell line in response to Aspergillus fumigatus hyphal fragments (HF) by cDNA microarray analysis, reverse transcriptase (RT) PCR, and enzyme-linked immunosorbent assay. Stimulation of THP-1 cells by HF alone caused a significant up-regulation of CCL4 (MIP1B) and CCL16, while CCL2 (MCP1) was down-regulated. By comparison, in the presence of VRC, a large number of genes such as CCL3 (MIP1A), CCL4 (MIP1B), CCL5 (RANTES), CCL7 (MCP3), CCL11 (EOTAXIN), CCL15 (MIP1Delta), CXCL6, and CXCL13 were strongly up-regulated in THP-1 cells challenged by HF, whereas CCL20 (MIP3A) and CCL21 (MIP2) were down-regulated. Among five genes differentially expressed in THP-1 cells, IL12A, IL12B, and IL-16 were down-regulated whereas IL-11 and TGFB1 were significantly up-regulated in the presence of VRC. The inflammation-related genes IFNgamma, IL1R1, and TNFA were also up-regulated in THP-1 cells exposed to HF only in the presence of VRC. RT-PCR of four selected genes validated the results of microarrays. The release of interleukin 1beta (IL-1beta) and IL-12 was significantly increased from monocytes stimulated either by HF alone (P < 0.05) or in the presence of VRC (P < 0.01 and P < 0.05, respectively). In contrast, tumor necrosis factor alpha release from monocytes was enhanced only in the presence of VRC (P < 0.01). The chemokines monocyte chemoattractant protein 1 and macrophage inflammatory protein 1beta were decreased under both conditions (P < 0.01). These results demonstrate that in the presence of VRC, HF induces a more pronounced profile of gene expression in THP-1 cells than HF alone, potentially leading to more-efficient host resistance to A. fumigatus. | lld:pubmed |
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| pubmed-article:17178797 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:17178797 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:17178797 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:17178797 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:17178797 | pubmed:issn | 0066-4804 | lld:pubmed |
| pubmed-article:17178797 | pubmed:author | pubmed-author:WalshT JTJ | lld:pubmed |
| pubmed-article:17178797 | pubmed:author | pubmed-author:OrfanosCC | lld:pubmed |
| pubmed-article:17178797 | pubmed:author | pubmed-author:PaliogianniFF | lld:pubmed |
| pubmed-article:17178797 | pubmed:author | pubmed-author:RoilidesEE | lld:pubmed |
| pubmed-article:17178797 | pubmed:author | pubmed-author:Simitsopoulou... | lld:pubmed |
| pubmed-article:17178797 | pubmed:author | pubmed-author:IoannidisJJ | lld:pubmed |
| pubmed-article:17178797 | pubmed:author | pubmed-author:LikartsisCC | lld:pubmed |
| pubmed-article:17178797 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:17178797 | pubmed:volume | 51 | lld:pubmed |
| pubmed-article:17178797 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:17178797 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:17178797 | pubmed:pagination | 1048-54 | lld:pubmed |
| pubmed-article:17178797 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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