| pubmed-article:17163906 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:17163906 | lifeskim:mentions | umls-concept:C0162741 | lld:lifeskim |
| pubmed-article:17163906 | lifeskim:mentions | umls-concept:C1510411 | lld:lifeskim |
| pubmed-article:17163906 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:17163906 | lifeskim:mentions | umls-concept:C0303920 | lld:lifeskim |
| pubmed-article:17163906 | lifeskim:mentions | umls-concept:C0336791 | lld:lifeskim |
| pubmed-article:17163906 | lifeskim:mentions | umls-concept:C0332219 | lld:lifeskim |
| pubmed-article:17163906 | lifeskim:mentions | umls-concept:C0872316 | lld:lifeskim |
| pubmed-article:17163906 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:17163906 | pubmed:dateCreated | 2006-12-13 | lld:pubmed |
| pubmed-article:17163906 | pubmed:abstractText | We demonstrate that fluorescent proteins can be used as visual selection markers for the transformation of Arabidopsis thaliana by the floral dip method. Seed-specific expression of green fluorescent protein (GFP) variants, as well as DsRed, permits the identification of mature transformed seeds in a large background of untransformed seeds by fluorescence microscopy. In planta visualization of transformed seeds in siliques shows that susceptibility to floral dip transformation is limited to a small, defined window in flower development. In the competent stage, the random transformation of up to 25% of the seeds within a single silique may occur. The use of fluorescent proteins with different spectral characteristics allows a rapid identification and genetic analysis of seeds that have received multiple genes-of-interest in co-transformation experiments. The data reveal that co-transformation does not occur at random, since the co-transformed genes are integrated at a single genetic locus in approximately 70% of the cases. This genetic linkage of the co-transformed genes greatly simplifies metabolic pathway engineering by reverse genetics in Arabidopsis. Additional advantages of using visual selection instead of antibiotic resistance include a rapid identification of the effect of the T-DNA insertion or the transgene on seed development and/or germination. This technology, of tagging and identifying transformed seeds by fluorescence provides a novel high-throughput screening system with many potential applications in plant biotechnology. | lld:pubmed |
| pubmed-article:17163906 | pubmed:language | eng | lld:pubmed |
| pubmed-article:17163906 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17163906 | pubmed:status | PubMed-not-MEDLINE | lld:pubmed |
| pubmed-article:17163906 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:17163906 | pubmed:issn | 1467-7652 | lld:pubmed |
| pubmed-article:17163906 | pubmed:author | pubmed-author:NapJan-PeterJ... | lld:pubmed |
| pubmed-article:17163906 | pubmed:author | pubmed-author:StuitjeAntoin... | lld:pubmed |
| pubmed-article:17163906 | pubmed:author | pubmed-author:KneppersTarci... | lld:pubmed |
| pubmed-article:17163906 | pubmed:author | pubmed-author:VerbreeElizab... | lld:pubmed |
| pubmed-article:17163906 | pubmed:author | pubmed-author:van der... | lld:pubmed |
| pubmed-article:17163906 | pubmed:author | pubmed-author:MietkiewskaEl... | lld:pubmed |
| pubmed-article:17163906 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:17163906 | pubmed:volume | 1 | lld:pubmed |
| pubmed-article:17163906 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:17163906 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:17163906 | pubmed:pagination | 301-9 | lld:pubmed |
| pubmed-article:17163906 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:17163906 | pubmed:articleTitle | Seed-expressed fluorescent proteins as versatile tools for easy (co)transformation and high-throughput functional genomics in Arabidopsis. | lld:pubmed |
| pubmed-article:17163906 | pubmed:affiliation | Department of Genetics, Institute for Molecular Celbiology, Vrije Universiteit, de Boelelaan 1087, 1081 HV, Amsterdam, The Netherlands. stuitje@bio.vu.nl | lld:pubmed |
| pubmed-article:17163906 | pubmed:publicationType | Journal Article | lld:pubmed |
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