| pubmed-article:17150804 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:17150804 | lifeskim:mentions | umls-concept:C0040711 | lld:lifeskim |
| pubmed-article:17150804 | lifeskim:mentions | umls-concept:C1327001 | lld:lifeskim |
| pubmed-article:17150804 | lifeskim:mentions | umls-concept:C0175921 | lld:lifeskim |
| pubmed-article:17150804 | lifeskim:mentions | umls-concept:C0185125 | lld:lifeskim |
| pubmed-article:17150804 | pubmed:issue | 50 | lld:pubmed |
| pubmed-article:17150804 | pubmed:dateCreated | 2006-12-7 | lld:pubmed |
| pubmed-article:17150804 | pubmed:abstractText | Here we describe a de novo tRNA acylation system consisting of artificial ribozymes. This system, the flexizyme (Fx) system, allows for the preparation of acyl-tRNAs with almost no limitation on the use of a variety of amino/hydroxy acids and tRNAs. To demonstrate its utility, we have carried out protein synthesis involving site-specific incorporation of nonnatural amino and hydroxy acids via amber or programmed frame-shift suppressions. We have also demonstrated peptide synthesis involving multiple nonnatural amino acids via sense codon suppression. The combination of the Fx system and appropriate cell-free translation is a powerful and flexible tool for mRNA-encoded synthesis of nonnatural polypeptides. | lld:pubmed |
| pubmed-article:17150804 | pubmed:language | eng | lld:pubmed |
| pubmed-article:17150804 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17150804 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:17150804 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17150804 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17150804 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17150804 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17150804 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17150804 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:17150804 | pubmed:issn | 1746-8272 | lld:pubmed |
| pubmed-article:17150804 | pubmed:author | pubmed-author:MurakamiHiros... | lld:pubmed |
| pubmed-article:17150804 | pubmed:author | pubmed-author:SugaHiroakiH | lld:pubmed |
| pubmed-article:17150804 | pubmed:author | pubmed-author:GotoYukiY | lld:pubmed |
| pubmed-article:17150804 | pubmed:author | pubmed-author:SakoYusukeY | lld:pubmed |
| pubmed-article:17150804 | pubmed:author | pubmed-author:OhtaAtushiA | lld:pubmed |
| pubmed-article:17150804 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:17150804 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:17150804 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:17150804 | pubmed:pagination | 35-6 | lld:pubmed |
| pubmed-article:17150804 | pubmed:dateRevised | 2007-9-19 | lld:pubmed |
| pubmed-article:17150804 | pubmed:meshHeading | pubmed-meshheading:17150804... | lld:pubmed |
| pubmed-article:17150804 | pubmed:meshHeading | pubmed-meshheading:17150804... | lld:pubmed |
| pubmed-article:17150804 | pubmed:meshHeading | pubmed-meshheading:17150804... | lld:pubmed |
| pubmed-article:17150804 | pubmed:meshHeading | pubmed-meshheading:17150804... | lld:pubmed |
| pubmed-article:17150804 | pubmed:meshHeading | pubmed-meshheading:17150804... | lld:pubmed |
| pubmed-article:17150804 | pubmed:meshHeading | pubmed-meshheading:17150804... | lld:pubmed |
| pubmed-article:17150804 | pubmed:meshHeading | pubmed-meshheading:17150804... | lld:pubmed |
| pubmed-article:17150804 | pubmed:meshHeading | pubmed-meshheading:17150804... | lld:pubmed |
| pubmed-article:17150804 | pubmed:meshHeading | pubmed-meshheading:17150804... | lld:pubmed |
| pubmed-article:17150804 | pubmed:meshHeading | pubmed-meshheading:17150804... | lld:pubmed |
| pubmed-article:17150804 | pubmed:year | 2006 | lld:pubmed |
| pubmed-article:17150804 | pubmed:articleTitle | Flexizyme as a versatile tRNA acylation catalyst and the application for translation. | lld:pubmed |
| pubmed-article:17150804 | pubmed:affiliation | Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan. | lld:pubmed |
| pubmed-article:17150804 | pubmed:publicationType | Journal Article | lld:pubmed |
