| pubmed-article:1714662 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1714662 | lifeskim:mentions | umls-concept:C0030657 | lld:lifeskim |
| pubmed-article:1714662 | lifeskim:mentions | umls-concept:C0019707 | lld:lifeskim |
| pubmed-article:1714662 | lifeskim:mentions | umls-concept:C0521026 | lld:lifeskim |
| pubmed-article:1714662 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:1714662 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:1714662 | pubmed:dateCreated | 1991-9-18 | lld:pubmed |
| pubmed-article:1714662 | pubmed:abstractText | The genomes of HIV and SIV are complex and contain several accessory genes which modulate viral replication and pathogenicity. One of these genes, vpx, is unique to the HIV-2/SIV group of viruses and encodes a virion-associated protein of unknown function. To examine the function of vpx, we constructed a vpx-deficient HIV-2 proviral clone and characterized its in vitro biological properties. Following transfection into immortalized T-cell lines, vpx-mutant HIV-2 was fully replication competent and exhibited growth kinetics and cytopathic properties equivalent to wild-type HIV-2. In addition, vpx-deficient virions were indistinguishable from wild-type HIV-2 in ultrastructure, composition of major structural proteins, and reverse transcriptase activity. In PHA-stimulated normal peripheral blood mononuclear cells (PBMCs), however, vpx-deficient virus replicated at substantially lower titers and required a 100- to 1000-fold higher inoculum to establish a productive infection. This defect was localized to early events in the viral life cycle since vpx-deficient virus exhibited a 5- to 10-fold reduction in initial (single cycle) viral DNA synthesis following acute infection of primary PBMCs. Paradoxically, in long-term (9-23 months) cultures of immortalized T-cells (SupT1) continuous high level replication of vpx-deficient, but not wild-type, virus was observed, indicating less efficient viral spread and cell killing and a more attenuated phenotype of vpx-deficient HIV-2. Taken together, these results demonstrate that vpx is required for the production of fully infectious and cytopathic HIV-2 virions and that it functions early in the viral life cycle by facilitating viral entry and/or reverse transcription. The pronounced replicative defect of vpx-deficient HIV-2 in primary PBMCs but not in short-term cultures of immortalized T-cell lines emphasizes the need to characterize the properties of nonessential HIV accessory gene products in natural target cells. | lld:pubmed |
| pubmed-article:1714662 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1714662 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1714662 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1714662 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:1714662 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1714662 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:1714662 | pubmed:issn | 0042-6822 | lld:pubmed |
| pubmed-article:1714662 | pubmed:author | pubmed-author:HahnB HBH | lld:pubmed |
| pubmed-article:1714662 | pubmed:author | pubmed-author:LeeS WSW | lld:pubmed |
| pubmed-article:1714662 | pubmed:author | pubmed-author:ShawG MGM | lld:pubmed |
| pubmed-article:1714662 | pubmed:author | pubmed-author:ConwayJ AJA | lld:pubmed |
| pubmed-article:1714662 | pubmed:author | pubmed-author:KappesJ CJC | lld:pubmed |
| pubmed-article:1714662 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1714662 | pubmed:volume | 184 | lld:pubmed |
| pubmed-article:1714662 | pubmed:geneSymbol | vpx | lld:pubmed |
| pubmed-article:1714662 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1714662 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1714662 | pubmed:pagination | 197-209 | lld:pubmed |
| pubmed-article:1714662 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
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| pubmed-article:1714662 | pubmed:year | 1991 | lld:pubmed |
| pubmed-article:1714662 | pubmed:articleTitle | Human immunodeficiency virus type 2 vpx protein augments viral infectivity. | lld:pubmed |
| pubmed-article:1714662 | pubmed:affiliation | Department of Medicine, University of Alabama, Birmingham 35294. | lld:pubmed |
| pubmed-article:1714662 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1714662 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:1714662 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
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