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pubmed-article:17120775pubmed:abstractTextMesenchymal stem cells (MSCs) provide an excellent source of pluripotent progenitor cells for tissue-engineering applications due to their proliferation capacity and differentiation potential. Genetic modification of MSCs with genes encoding tissue-specific growth factors and cytokines can induce and maintain lineage-specific differentiation. Due to anatomical and physiological similarities to humans, porcine research models have been proven valuable for the preclinical testing of tissue engineering protocols in large animals. The aim of this study was to evaluate optimized viral and non-viral ex vivo gene delivery systems with respect to gene transfer efficiency, maintenance of transgene expression, and safety issues using primary porcine MSCs as target cells.lld:pubmed
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pubmed-article:17120775pubmed:dateRevised2008-11-21lld:pubmed
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pubmed-article:17120775pubmed:year2006lld:pubmed
pubmed-article:17120775pubmed:articleTitleOptimizing viral and non-viral gene transfer methods for genetic modification of porcine mesenchymal stem cells.lld:pubmed
pubmed-article:17120775pubmed:affiliationOrthopaedic Research Laboratory, Department of Orthopaedic Surgery E, Aarhus University Hospital, Aarhus, Denmark. maik.stiehler@ki.au.dklld:pubmed
pubmed-article:17120775pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:17120775pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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