| pubmed-article:17105240 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:17105240 | lifeskim:mentions | umls-concept:C0085104 | lld:lifeskim |
| pubmed-article:17105240 | lifeskim:mentions | umls-concept:C0028132 | lld:lifeskim |
| pubmed-article:17105240 | lifeskim:mentions | umls-concept:C0220781 | lld:lifeskim |
| pubmed-article:17105240 | lifeskim:mentions | umls-concept:C1883254 | lld:lifeskim |
| pubmed-article:17105240 | lifeskim:mentions | umls-concept:C0185125 | lld:lifeskim |
| pubmed-article:17105240 | lifeskim:mentions | umls-concept:C0936012 | lld:lifeskim |
| pubmed-article:17105240 | lifeskim:mentions | umls-concept:C0301869 | lld:lifeskim |
| pubmed-article:17105240 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:17105240 | pubmed:dateCreated | 2006-11-19 | lld:pubmed |
| pubmed-article:17105240 | pubmed:abstractText | High-affinity nitrilotriacetic acids (NTA) have great potential in the molecular manipulation of His-tagged proteins. We have developed a facile method to synthesize multivalent NTA and its conjugates. Starting with appropriately protected lysine, we synthesized the mono-NTA synthons functionalized with either an amino group or a carboxylic group. We then obtained tri-NTA through the condensation of the amino NTA and the carboxylic NTA. Using amino tri-NTA as the key intermediate, we synthesized a series of tri-NTA conjugates with a variety of functional units including biotin, dialkyl, fluorescein, and a hydroxybenzimidate moiety. The biotin-tri-NTA was employed to convert a Biacore streptavidin chip into a high-affinity tri-NTA chip. The equilibrium dissociation constants of tri-NTA/His-tagged protein complexes measured by surface plasmon resonance are in the 20 nM range. Histidine(6)-tagged yeast cytosine deaminase (His6-yCD) was incorporated onto the liposome surface by the lipid-tri-NTA conjugate without any activity loss. Fluorescein-tri-NTA formed a stable 1:1 complex with His6-yCD without significant fluorescence quenching. Specific tri-NTA derivatives for the radiolabeling and coupling of two His-tagged proteins to each other are described. Thus, we have added to the toolbox a number of high-affinity tri-NTA adaptors for the manipulation of His-tagged molecules. | lld:pubmed |
| pubmed-article:17105240 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17105240 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17105240 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17105240 | pubmed:language | eng | lld:pubmed |
| pubmed-article:17105240 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17105240 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:17105240 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17105240 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17105240 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17105240 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17105240 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17105240 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:17105240 | pubmed:issn | 1043-1802 | lld:pubmed |
| pubmed-article:17105240 | pubmed:author | pubmed-author:SzokaFrancis... | lld:pubmed |
| pubmed-article:17105240 | pubmed:author | pubmed-author:ParkJoshua... | lld:pubmed |
| pubmed-article:17105240 | pubmed:author | pubmed-author:HwangPeterP | lld:pubmed |
| pubmed-article:17105240 | pubmed:author | pubmed-author:WatsonDouglas... | lld:pubmed |
| pubmed-article:17105240 | pubmed:author | pubmed-author:HuangZhaohuaZ | lld:pubmed |
| pubmed-article:17105240 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:17105240 | pubmed:volume | 17 | lld:pubmed |
| pubmed-article:17105240 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:17105240 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:17105240 | pubmed:pagination | 1592-600 | lld:pubmed |
| pubmed-article:17105240 | pubmed:dateRevised | 2007-12-3 | lld:pubmed |
| pubmed-article:17105240 | pubmed:meshHeading | pubmed-meshheading:17105240... | lld:pubmed |
| pubmed-article:17105240 | pubmed:meshHeading | pubmed-meshheading:17105240... | lld:pubmed |
| pubmed-article:17105240 | pubmed:meshHeading | pubmed-meshheading:17105240... | lld:pubmed |
| pubmed-article:17105240 | pubmed:meshHeading | pubmed-meshheading:17105240... | lld:pubmed |
| pubmed-article:17105240 | pubmed:meshHeading | pubmed-meshheading:17105240... | lld:pubmed |
| pubmed-article:17105240 | pubmed:meshHeading | pubmed-meshheading:17105240... | lld:pubmed |
| pubmed-article:17105240 | pubmed:meshHeading | pubmed-meshheading:17105240... | lld:pubmed |
| pubmed-article:17105240 | pubmed:articleTitle | Facile synthesis of multivalent nitrilotriacetic acid (NTA) and NTA conjugates for analytical and drug delivery applications. | lld:pubmed |
| pubmed-article:17105240 | pubmed:affiliation | Department of Pharmaceutical Chemistry, School of Pharmacy, University of California at San Francisco, San Francisco, California 94143-0446, USA. | lld:pubmed |
| pubmed-article:17105240 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:17105240 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:17105240 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
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